Hypoxic effects on the expression of mineralocorticoid and glucocorticoid receptors in human renal cortex epithelial cells

W. Jenq, Hamid Rabb, M. Wahe, G. Ramirez

Research output: Contribution to journalArticle

Abstract

The effects of both mild and severe hypoxic conditions on the expression of mineralocorticoid receptor (MR) and glucocorticoid receptor (GR) in the primary human renal cortex epithelial cells (RCEC) were investigated. Slot blot analyses were performed with human β-actin cDNA and the cDNA probes for unique regions of MR and GR. The steady-state mRNA level of the house-keeping β-actin gene remains the same in RCEC under either normoxic or hypoxic conditions. However, there was a 45% reduction in MR expression and a 30% increase in GR expression, under mild hypoxic condition (15% O2,) over 3 days. Furthermore, cells subjected to a severe hypoxic condition (0.5% O2,) for 24 hours exerted a 22% reduction in MR expression and a 64% increase in GR expression. Changes in MR and GR expression may, in part, explain the physiological changes in adrenal cortical function observed in hypoxic conditions.

Original languageEnglish (US)
Pages (from-to)444-448
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume218
Issue number2
DOIs
StatePublished - Jan 17 1996
Externally publishedYes

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Mineralocorticoid Receptors
Glucocorticoid Receptors
Epithelial Cells
Kidney
Actins
Complementary DNA
Essential Genes
Genes
Messenger RNA

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Hypoxic effects on the expression of mineralocorticoid and glucocorticoid receptors in human renal cortex epithelial cells. / Jenq, W.; Rabb, Hamid; Wahe, M.; Ramirez, G.

In: Biochemical and Biophysical Research Communications, Vol. 218, No. 2, 17.01.1996, p. 444-448.

Research output: Contribution to journalArticle

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