Hypoxia-inducible nuclear factors bind to an enhancer element located 3′ to the human erythropoietin gene

Gregg L. Semenza, Mary K. Nejfelt, Suzie M. Chi, Stylianos E. Antonarakis

Research output: Contribution to journalArticle

Abstract

Human erythropoietin gene expression in liver and kidney is inducible by anemia or hypoxia. DNase I-hypersensitive sites were identified 3′ to the human erythropoietin gene in liver nuclei. A 256-base-pair region of 3′ flanking sequence was shown by DNase I protection and electrophoretic mobility-shift assays to bind four or more different nuclear factors, at least two of which are induced by anemia in both liver and kidney, and the region functioned as a hypoxia-inducible enhancer in transient expression assays. These results provide insight into the molecular basis for the regulation of gene expression by a fundamental physiologic stimulus, hypoxia.

Original languageEnglish (US)
Pages (from-to)5680-5684
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number13
DOIs
StatePublished - Jul 1 1991

ASJC Scopus subject areas

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