Hypoxia induced the remodeling of pulmonary vein smooth muscle and reduced peroxisome proliferator activated receptor γ expression

Jie Zhang, Qian Jiang, Xiuqing Chen, Yuqin Chen, Wenju Lu, Jian Wang

Research output: Contribution to journalArticlepeer-review

Abstract

OBJECTIVE: To investigate the effect of chronic hypoxia on pulmonary vein remodeling in rats and its underlying molecular mechanism.

METHODS: Totally 20 adult SD rats (200-250 g), in accordance with a random number table, were divided into normoxia control group (NC) and chronic hypoxia group (CH). CH group rats were put into automatic hypoxia box for 21 days. After that, RVSP, mRVP and RV/( LV+S) were measured; Lung histopathological sections were prepared. The lumen area, ratio of wall thickness to radius of pulmonary veins were gauged by using the Image Pro Plus 6.0 software. Primary PVSMCs were cultured in hypoxia (4% O₂) or normoxia (21% O₂) for 60 hours respectively before extracted total protein for Western blotting analysis. PPARγ protein expression in rat pulmonary vein smooth muscle and pulmonary vein smooth muscle cells was detected by Western blot.

RESULTS: Compared with NC group [(27.06 ± 2.10), (12.58 ± 0.82) mmHg (1 mmHg=0.133 kPa)], the RVSP and mRVP in CH group [(50.46 ± 9.51), (21.66 ± 1.85)mmHg] were significantly increased (P

CONCLUSION: Hypoxia induced increase of RVSP, mRVP, RV/(LV+S), and accompanied with pulmonary venous remodeling. The underlying mechanism of the vein change may related to down-regulated expression of PPARγ.

Original languageEnglish (US)
Pages (from-to)607-611
Number of pages5
JournalZhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases
Volume38
Issue number8
StatePublished - Aug 1 2015
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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