Hypoxia-induced reporter genes with different half-lives

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The utility of reporter genes has gained significant momentum over the last three decades. Reporter genes are used to understand the transcriptional activity of a gene both in vitro and in vivo, and in pathway analysis and drug screening for diseases involving protozoan parasites, and in anti-cancer drug developments. Here, using a human prostate cancer xenograft model (PC3), we describe a method to construct and validate hypoxia reporter genes with different half-lives. Using molecular biology and optical imaging techniques, we have validated the expression of long half-life enhanced green fluorescence protein (EGFP) expression and short half-life luciferase gene expression to report on the spatial and temporal evolution of hypoxia in vivo.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages113-125
Number of pages13
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1790
ISSN (Print)1064-3745

Fingerprint

Reporter Genes
Half-Life
Preclinical Drug Evaluations
Molecular Imaging
Optical Imaging
Luciferases
Heterografts
Molecular Biology
Prostatic Neoplasms
Parasites
Fluorescence
Gene Expression
Pharmaceutical Preparations
Genes
Hypoxia
Neoplasms
Proteins

Keywords

  • Bioluminescence
  • Hypoxia
  • Hypoxia response elements (HRE)
  • Lentivirus
  • Luciferase assay
  • Reporter gene

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Krishnamachary, B., Danhier, P., Kakkad, S., Bharti, S., & Bhujwalla, Z. M. (2018). Hypoxia-induced reporter genes with different half-lives. In Methods in Molecular Biology (pp. 113-125). (Methods in Molecular Biology; Vol. 1790). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7860-1_9

Hypoxia-induced reporter genes with different half-lives. / Krishnamachary, Balaji; Danhier, Pierre; Kakkad, Samata; Bharti, Santosh; Bhujwalla, Zaver M.

Methods in Molecular Biology. Humana Press Inc., 2018. p. 113-125 (Methods in Molecular Biology; Vol. 1790).

Research output: Chapter in Book/Report/Conference proceedingChapter

Krishnamachary, B, Danhier, P, Kakkad, S, Bharti, S & Bhujwalla, ZM 2018, Hypoxia-induced reporter genes with different half-lives. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1790, Humana Press Inc., pp. 113-125. https://doi.org/10.1007/978-1-4939-7860-1_9
Krishnamachary B, Danhier P, Kakkad S, Bharti S, Bhujwalla ZM. Hypoxia-induced reporter genes with different half-lives. In Methods in Molecular Biology. Humana Press Inc. 2018. p. 113-125. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7860-1_9
Krishnamachary, Balaji ; Danhier, Pierre ; Kakkad, Samata ; Bharti, Santosh ; Bhujwalla, Zaver M. / Hypoxia-induced reporter genes with different half-lives. Methods in Molecular Biology. Humana Press Inc., 2018. pp. 113-125 (Methods in Molecular Biology).
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