Hypoxia-induced reporter genes with different half-lives

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The utility of reporter genes has gained significant momentum over the last three decades. Reporter genes are used to understand the transcriptional activity of a gene both in vitro and in vivo, and in pathway analysis and drug screening for diseases involving protozoan parasites, and in anti-cancer drug developments. Here, using a human prostate cancer xenograft model (PC3), we describe a method to construct and validate hypoxia reporter genes with different half-lives. Using molecular biology and optical imaging techniques, we have validated the expression of long half-life enhanced green fluorescence protein (EGFP) expression and short half-life luciferase gene expression to report on the spatial and temporal evolution of hypoxia in vivo.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages113-125
Number of pages13
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1790
ISSN (Print)1064-3745

Keywords

  • Bioluminescence
  • Hypoxia
  • Hypoxia response elements (HRE)
  • Lentivirus
  • Luciferase assay
  • Reporter gene

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Krishnamachary, B., Danhier, P., Kakkad, S., Bharti, S., & Bhujwalla, Z. M. (2018). Hypoxia-induced reporter genes with different half-lives. In Methods in Molecular Biology (pp. 113-125). (Methods in Molecular Biology; Vol. 1790). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7860-1_9