Hypoxia-induced mitogenic factor modulates surfactant protein B and C expression in mouse lung

Qiangsong Tong, Liduan Zheng, Jeffrey M Dodd-o, John Langer, Danming Wang, Dechun Li

Research output: Contribution to journalArticle

Abstract

Previous studies have demonstrated a robust pulmonary expression of hypoxia-induced mitogenic factor (HIMF) during the perinatal period, when surfactant protein (SP) synthesis begins. We hypothesized that HIMF modulates SP expression and participates in lung development and maturation. The temporal-spatial expression of HIMF, SP-B, and SP-C in developing mouse lungs was examined by immunohistochemical staining, Western blot, and RT-PCR. The expression and localization of SP-B and SP-C were investigated in mouse lungs after intratracheal instillation of HIMF in adult mice. The effects of HIMF on SP-B and SP-C transcription activity, and on mRNA degradation, were investigated in mouse lung epithelial (MLE)-12 and C10 cells using the promoter-luciferase reporter assay and actinomycin D incubation. The activation of Akt, extracellular signal-regulated kinase (ERK)1/2, and p38 mitogen-activated protein kinase was explored by Western blot. Intratracheal instillation of HIMF resulted in significant increases of SP-B and SP-C production, predominantly localized to alveolar type II cells. In MLE-12 and C10 cells, HIMF enhanced SP-B and SP-C mRNA levels in a dose-dependent manner. Meanwhile, HIMF increased transcription activity and prevented actinomycin D-facilitated SP-B and SP-C mRNA degradation in MLE-12 cells. Incubation of cells with LY294002, PD098059, or U0126 abolished HIMF-induced Akt and ERKI/2 phosphorylation and suppressed HIMF-induced SP-B and SP-C production, whereas SB203580 had no effect. These results indicate that HIMF induces SP-B and SP-C production in mouse lungs and alveolar type II-like cell lines via activations of phosphatidylinositol 3-kinase/Akt and ERK1/2 mitogen-activated protein kinase, suggesting that HIMF plays critical roles in lung development and maturation.

Original languageEnglish (US)
Pages (from-to)28-38
Number of pages11
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume34
Issue number1
DOIs
StatePublished - Jan 2006
Externally publishedYes

Fingerprint

Protein C
Surface-Active Agents
Lung
IgA receptor
Hypoxia
Mitogen-Activated Protein Kinase 1
RNA Stability
Dactinomycin
Messenger RNA
Western Blotting
Chemical activation
Cells
Phosphatidylinositol 3-Kinase
Alveolar Epithelial Cells
Cell Hypoxia
Degradation
Phosphorylation
2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
Transcription factors
Mitogen-Activated Protein Kinase 3

Keywords

  • Gene expression
  • Lung development
  • Lung epithelial cells
  • mRNA stability
  • Signal transduction

ASJC Scopus subject areas

  • Cell Biology
  • Pulmonary and Respiratory Medicine
  • Molecular Biology

Cite this

Hypoxia-induced mitogenic factor modulates surfactant protein B and C expression in mouse lung. / Tong, Qiangsong; Zheng, Liduan; Dodd-o, Jeffrey M; Langer, John; Wang, Danming; Li, Dechun.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 34, No. 1, 01.2006, p. 28-38.

Research output: Contribution to journalArticle

Tong, Qiangsong ; Zheng, Liduan ; Dodd-o, Jeffrey M ; Langer, John ; Wang, Danming ; Li, Dechun. / Hypoxia-induced mitogenic factor modulates surfactant protein B and C expression in mouse lung. In: American Journal of Respiratory Cell and Molecular Biology. 2006 ; Vol. 34, No. 1. pp. 28-38.
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