Hypoxia enhances proliferation and tissue formation of human mesenchymal stem cells

Warren L Grayson, Feng Zhao, Bruce Bunnell, Teng Ma

Research output: Contribution to journalArticle

Abstract

Changes in oxygen concentrations affect many of the innate characteristics of stem and progenitor cells. Human mesenchymal stem cells (hMSCs) were maintained under hypoxic atmospheres (2% O2) for up to seven in vitro passages. This resulted in approximately 30-fold higher hMSC expansion over 6 weeks without loss of multi-lineage differentiation capabilities. Under hypoxia, hMSCs maintained their growth-rates even after reaching confluence, resulting in the formation of multiple cell layers. Hypoxic hMSCs also displayed differences in the cell and nuclear morphologies as well as enhanced ECM formation and organization. These changes in cellular characteristics were accompanied by higher mRNA levels of Oct-4 and HIF-2α, as well as increased expression levels of connexin-43, a protein used in gap junction formation. The results from this study demonstrated that oxygen concentrations affected many aspects of stem-cell physiology, including growth and in vitro development, and may be a critical parameter during expansion and differentiation.

Original languageEnglish (US)
Pages (from-to)948-953
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume358
Issue number3
DOIs
StatePublished - Jul 6 2007
Externally publishedYes

Fingerprint

Stem cells
Mesenchymal Stromal Cells
Tissue
Stem Cells
Oxygen
Cell Physiological Phenomena
Connexin 43
Gap Junctions
Military electronic countermeasures
Growth
Atmosphere
Physiology
Messenger RNA
Cells
Hypoxia
Proteins
In Vitro Techniques

Keywords

  • Connexins
  • Differentiation
  • ECM
  • HIF-2
  • hMSCs
  • Hypoxia
  • Oct-4
  • Proliferation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Hypoxia enhances proliferation and tissue formation of human mesenchymal stem cells. / Grayson, Warren L; Zhao, Feng; Bunnell, Bruce; Ma, Teng.

In: Biochemical and Biophysical Research Communications, Vol. 358, No. 3, 06.07.2007, p. 948-953.

Research output: Contribution to journalArticle

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