Hypoxia and hypoxia-inducible factor in the burn wound

Dongmei Xing, Lixin Liu, Guy P. Marti, Xianjie Zhang, Maura Reinblatt, Stephen M. Milner, John Harmon

Research output: Contribution to journalArticle

Abstract

The importance of hypoxia-inducible factor (HIF) in promoting angiogenesis and vasculogenesis during wound healing has been demonstrated. It is widely accepted that HIF activity can be promoted by many factors, including hypoxia in the wound or cytokines from inflammatory cells infiltrating the wound. However, there has not been a systematic exploration of the relationship between HIF activity and hypoxia in the burn wound. The location of the hypoxic tissue has not been clearly delineated. The time course of the appearance of hypoxia and the increased activity of HIF and appearance of HIF's downstream transcription products has not been described. The aim of this study was to utilize pimonidazole, a specific tissue hypoxia marker, to characterize the spatial and temporal course of hypoxia in a murine burn model and correlate this with the appearance of HIF-1α and its important angiogenic and vasculogenic transcription products vascular endothelial growth factor and SDF-1. Hypoxia was found in the healing margin of burn wounds beginning at 48 hours after burn and peaking at day 3 after burn. On sequential sections of the same tissue block, positive staining of HIF-1α, SDF-1, and vascular endothelial growth factor all occurred at the leading margin of the healing area and peaked at day 3, as did hypoxia. Immunohistochemical analysis was used to explore the characteristics of the hypoxic region of the wound. The localization of hypoxia was found to be related to cell growth and migration, but not to proliferation or inflammatory infiltration.

Original languageEnglish (US)
Pages (from-to)205-213
Number of pages9
JournalWound Repair and Regeneration
Volume19
Issue number2
DOIs
StatePublished - Mar 2011

Fingerprint

Wounds and Injuries
Hypoxia-Inducible Factor 1
Vascular Endothelial Growth Factor A
Hypoxia
Wound Healing
Cell Movement
Staining and Labeling
Cytokines
Growth

ASJC Scopus subject areas

  • Dermatology
  • Surgery

Cite this

Xing, D., Liu, L., Marti, G. P., Zhang, X., Reinblatt, M., Milner, S. M., & Harmon, J. (2011). Hypoxia and hypoxia-inducible factor in the burn wound. Wound Repair and Regeneration, 19(2), 205-213. https://doi.org/10.1111/j.1524-475X.2010.00656.x

Hypoxia and hypoxia-inducible factor in the burn wound. / Xing, Dongmei; Liu, Lixin; Marti, Guy P.; Zhang, Xianjie; Reinblatt, Maura; Milner, Stephen M.; Harmon, John.

In: Wound Repair and Regeneration, Vol. 19, No. 2, 03.2011, p. 205-213.

Research output: Contribution to journalArticle

Xing, D, Liu, L, Marti, GP, Zhang, X, Reinblatt, M, Milner, SM & Harmon, J 2011, 'Hypoxia and hypoxia-inducible factor in the burn wound', Wound Repair and Regeneration, vol. 19, no. 2, pp. 205-213. https://doi.org/10.1111/j.1524-475X.2010.00656.x
Xing D, Liu L, Marti GP, Zhang X, Reinblatt M, Milner SM et al. Hypoxia and hypoxia-inducible factor in the burn wound. Wound Repair and Regeneration. 2011 Mar;19(2):205-213. https://doi.org/10.1111/j.1524-475X.2010.00656.x
Xing, Dongmei ; Liu, Lixin ; Marti, Guy P. ; Zhang, Xianjie ; Reinblatt, Maura ; Milner, Stephen M. ; Harmon, John. / Hypoxia and hypoxia-inducible factor in the burn wound. In: Wound Repair and Regeneration. 2011 ; Vol. 19, No. 2. pp. 205-213.
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