TY - JOUR
T1 - Hyperspectral Imaging (HSI) of Human Kidney Allografts
AU - Sucher, Robert
AU - Wagner, Tristan
AU - Köhler, Hannes
AU - Sucher, Elisabeth
AU - Quice, Hanna
AU - Recknagel, Sebastian
AU - Lederer, Andri
AU - Hau, Hans Michael
AU - Rademacher, Sebastian
AU - Schneeberger, Stefan
AU - Brandacher, Gerald
AU - Qockel, Ines
AU - Seehofer, Daniel
N1 - Publisher Copyright:
© 2022 Lippincott Williams and Wilkins. All rights reserved.
PY - 2022/7/1
Y1 - 2022/7/1
N2 - Objective:Aim of our study was to test a noninvasive HSI technique as an intraoperative real time assessment tool for deceased donor kidney quality and function in human kidney allotransplantation.Summary of Background Data:HSI is capable to deliver quantitative diagnostic information about tissue pathology, morphology, and composition, based on the spectral characteristics of the investigated tissue. Because tools for objective intraoperative graft viability and performance assessment are lacking, we applied this novel technique to human kidney transplantation.Methods:Hyperspectral images of distinct components of kidney allografts (parenchyma, ureter) were acquired 15 and 45 minutes after reperfusion and subsequently analyzed using specialized HSI acquisition software capable to compute oxygen saturation levels (StO2), near infrared perfusion indices (NIR), organ hemoglobin indices, and tissue water indices of explored tissues.Results:Seventeen kidney transplants were analyzed. Median recipient and donor age were 55-years. Cold ischemia time was 10.8-±-4.1-hours and anastomosis time was 35-±-7-minutes (mean-±-standard deviation). Two patients (11.8%) developed delayed graft function (DGF). cold ischemia time was significantly longer (18.6-±-1.6) in patients with DGF (P < 0.01). Kidneys with DGF furthermore displayed significant lower StO2(P = 0.02) and NIR perfusion indices, 15 minutes after reperfusion (P < 0.01). Transplant ureters displayed a significant decrease of NIR perfusion with increased distance to the renal pelvis, identifying well and poor perfused segments.Conclusion:Intraoperative HSI is feasible and meaningful to predict DGF in renal allografts. Furthermore, it can be utilized for image guided surgery, providing information about tissue oxygenation, perfusion, hemoglobin concentration, and water concentration, hence allowing intraoperative viability assessment of the kidney parenchyma and the ureter.
AB - Objective:Aim of our study was to test a noninvasive HSI technique as an intraoperative real time assessment tool for deceased donor kidney quality and function in human kidney allotransplantation.Summary of Background Data:HSI is capable to deliver quantitative diagnostic information about tissue pathology, morphology, and composition, based on the spectral characteristics of the investigated tissue. Because tools for objective intraoperative graft viability and performance assessment are lacking, we applied this novel technique to human kidney transplantation.Methods:Hyperspectral images of distinct components of kidney allografts (parenchyma, ureter) were acquired 15 and 45 minutes after reperfusion and subsequently analyzed using specialized HSI acquisition software capable to compute oxygen saturation levels (StO2), near infrared perfusion indices (NIR), organ hemoglobin indices, and tissue water indices of explored tissues.Results:Seventeen kidney transplants were analyzed. Median recipient and donor age were 55-years. Cold ischemia time was 10.8-±-4.1-hours and anastomosis time was 35-±-7-minutes (mean-±-standard deviation). Two patients (11.8%) developed delayed graft function (DGF). cold ischemia time was significantly longer (18.6-±-1.6) in patients with DGF (P < 0.01). Kidneys with DGF furthermore displayed significant lower StO2(P = 0.02) and NIR perfusion indices, 15 minutes after reperfusion (P < 0.01). Transplant ureters displayed a significant decrease of NIR perfusion with increased distance to the renal pelvis, identifying well and poor perfused segments.Conclusion:Intraoperative HSI is feasible and meaningful to predict DGF in renal allografts. Furthermore, it can be utilized for image guided surgery, providing information about tissue oxygenation, perfusion, hemoglobin concentration, and water concentration, hence allowing intraoperative viability assessment of the kidney parenchyma and the ureter.
KW - delayed graft function
KW - hyperspectral imaging
KW - kidney transplantation
UR - http://www.scopus.com/inward/record.url?scp=85133240104&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85133240104&partnerID=8YFLogxK
U2 - 10.1097/SLA.0000000000004429
DO - 10.1097/SLA.0000000000004429
M3 - Article
C2 - 33196483
AN - SCOPUS:85133240104
SN - 0003-4932
VL - 276
SP - E48-E55
JO - Annals of surgery
JF - Annals of surgery
IS - 1
ER -