Hyperosmotic arabinose solutions open the tight junctions between brain capillary endothelial cells in tissue culture

Katerina Dorovini-Zis; Phillip D. Bowman; A. Lorris Betz; Gary W. Goldstein

doi:10.1016/0006-8993(84)90254-3

Hyperosmotic arabinose solutions open the tight junctions between brain capillary endothelial cells in tissue culture

Katerina Dorovini-Zis, Phillip D. Bowman, A. Lorris Betz, Gary W. Goldstein

Research output: Contribution to journal › Article › peer-review

Abstract

Tight junctions between bovine brain capillary endothelial cells in primary cell culture are impermeable to horseradish peroxidase (HRP) after 5 min of incubation. However, following 1-5 min of exposure to 1.6 M arabinose, HRP penetrates the extracellular space between successive tight junctions. Endothelial cells in control cultures contain a small number of cytoplasmic pits and vesicles containing HRP which do not increase in number after hyperosmotic treatment.

Original language	English (US)
Pages (from-to)	383-386
Number of pages	4
Journal	Brain research
Volume	302
Issue number	2
DOIs	https://doi.org/10.1016/0006-8993(84)90254-3
State	Published - Jun 8 1984
Externally published	Yes

Keywords

arabinose
brain capillary endothelium
horseradish peroxidase
tight junctions

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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abstract = "Tight junctions between bovine brain capillary endothelial cells in primary cell culture are impermeable to horseradish peroxidase (HRP) after 5 min of incubation. However, following 1-5 min of exposure to 1.6 M arabinose, HRP penetrates the extracellular space between successive tight junctions. Endothelial cells in control cultures contain a small number of cytoplasmic pits and vesicles containing HRP which do not increase in number after hyperosmotic treatment.",

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AU - Goldstein, Gary W.

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KW - tight junctions

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