Hyperosmotic arabinose solutions open the tight junctions between brain capillary endothelial cells in tissue culture

Katerina Dorovini-Zis; Phillip D. Bowman; A. Lorris Betz; Gary W. Goldstein

doi:10.1016/0006-8993(84)90254-3

Hyperosmotic arabinose solutions open the tight junctions between brain capillary endothelial cells in tissue culture

Katerina Dorovini-Zis, Phillip D. Bowman, A. Lorris Betz, Gary W. Goldstein

Research output: Contribution to journal › Article › peer-review

Abstract

Tight junctions between bovine brain capillary endothelial cells in primary cell culture are impermeable to horseradish peroxidase (HRP) after 5 min of incubation. However, following 1-5 min of exposure to 1.6 M arabinose, HRP penetrates the extracellular space between successive tight junctions. Endothelial cells in control cultures contain a small number of cytoplasmic pits and vesicles containing HRP which do not increase in number after hyperosmotic treatment.

Original language	English (US)
Pages (from-to)	383-386
Number of pages	4
Journal	Brain research
Volume	302
Issue number	2
DOIs	https://doi.org/10.1016/0006-8993(84)90254-3
State	Published - Jun 8 1984
Externally published	Yes

Keywords

arabinose
brain capillary endothelium
horseradish peroxidase
tight junctions

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/0006-8993(84)90254-3

Cite this

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title = "Hyperosmotic arabinose solutions open the tight junctions between brain capillary endothelial cells in tissue culture",

abstract = "Tight junctions between bovine brain capillary endothelial cells in primary cell culture are impermeable to horseradish peroxidase (HRP) after 5 min of incubation. However, following 1-5 min of exposure to 1.6 M arabinose, HRP penetrates the extracellular space between successive tight junctions. Endothelial cells in control cultures contain a small number of cytoplasmic pits and vesicles containing HRP which do not increase in number after hyperosmotic treatment.",

keywords = "arabinose, brain capillary endothelium, horseradish peroxidase, tight junctions",

author = "Katerina Dorovini-Zis and Bowman, {Phillip D.} and {Lorris Betz}, A. and Goldstein, {Gary W.}",

note = "Funding Information: This study was supportedb y a TeacherI nvestiga-tor Award (1K07NS00708f)r om the National Institutes of Health and by a Faculty ResearchG rant from the Rackham School of Graduate Studies (K.D.-Z.) and by the National Foundation-March of Dimes and Grants HL-25492 and ES-02380f rom the National Instituteso f Health. A.L.B. is the recipienot f an EstablisheIdn vestigatorshfirpo m the AmericanH eartAssociation.",

year = "1984",

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doi = "10.1016/0006-8993(84)90254-3",

language = "English (US)",

volume = "302",

pages = "383--386",

journal = "Brain Research",

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T1 - Hyperosmotic arabinose solutions open the tight junctions between brain capillary endothelial cells in tissue culture

AU - Dorovini-Zis, Katerina

AU - Bowman, Phillip D.

AU - Lorris Betz, A.

AU - Goldstein, Gary W.

N1 - Funding Information: This study was supportedb y a TeacherI nvestiga-tor Award (1K07NS00708f)r om the National Institutes of Health and by a Faculty ResearchG rant from the Rackham School of Graduate Studies (K.D.-Z.) and by the National Foundation-March of Dimes and Grants HL-25492 and ES-02380f rom the National Instituteso f Health. A.L.B. is the recipienot f an EstablisheIdn vestigatorshfirpo m the AmericanH eartAssociation.

PY - 1984/6/8

Y1 - 1984/6/8

N2 - Tight junctions between bovine brain capillary endothelial cells in primary cell culture are impermeable to horseradish peroxidase (HRP) after 5 min of incubation. However, following 1-5 min of exposure to 1.6 M arabinose, HRP penetrates the extracellular space between successive tight junctions. Endothelial cells in control cultures contain a small number of cytoplasmic pits and vesicles containing HRP which do not increase in number after hyperosmotic treatment.

AB - Tight junctions between bovine brain capillary endothelial cells in primary cell culture are impermeable to horseradish peroxidase (HRP) after 5 min of incubation. However, following 1-5 min of exposure to 1.6 M arabinose, HRP penetrates the extracellular space between successive tight junctions. Endothelial cells in control cultures contain a small number of cytoplasmic pits and vesicles containing HRP which do not increase in number after hyperosmotic treatment.

KW - arabinose

KW - brain capillary endothelium

KW - horseradish peroxidase

KW - tight junctions

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Hyperosmotic arabinose solutions open the tight junctions between brain capillary endothelial cells in tissue culture

Abstract

Keywords

ASJC Scopus subject areas

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