Abstract
Tight junctions between bovine brain capillary endothelial cells in primary cell culture are impermeable to horseradish peroxidase (HRP) after 5 min of incubation. However, following 1-5 min of exposure to 1.6 M arabinose, HRP penetrates the extracellular space between successive tight junctions. Endothelial cells in control cultures contain a small number of cytoplasmic pits and vesicles containing HRP which do not increase in number after hyperosmotic treatment.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 383-386 |
| Number of pages | 4 |
| Journal | Brain Research |
| Volume | 302 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jun 8 1984 |
| Externally published | Yes |
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Keywords
- arabinose
- brain capillary endothelium
- horseradish peroxidase
- tight junctions
ASJC Scopus subject areas
- Developmental Biology
- Molecular Biology
- Clinical Neurology
- Neuroscience(all)
Cite this
Hyperosmotic arabinose solutions open the tight junctions between brain capillary endothelial cells in tissue culture. / Dorovini-Zis, Katerina; Bowman, Phillip D.; Lorris Betz, A.; Goldstein, Gary William.
In: Brain Research, Vol. 302, No. 2, 08.06.1984, p. 383-386.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Hyperosmotic arabinose solutions open the tight junctions between brain capillary endothelial cells in tissue culture
AU - Dorovini-Zis, Katerina
AU - Bowman, Phillip D.
AU - Lorris Betz, A.
AU - Goldstein, Gary William
PY - 1984/6/8
Y1 - 1984/6/8
N2 - Tight junctions between bovine brain capillary endothelial cells in primary cell culture are impermeable to horseradish peroxidase (HRP) after 5 min of incubation. However, following 1-5 min of exposure to 1.6 M arabinose, HRP penetrates the extracellular space between successive tight junctions. Endothelial cells in control cultures contain a small number of cytoplasmic pits and vesicles containing HRP which do not increase in number after hyperosmotic treatment.
AB - Tight junctions between bovine brain capillary endothelial cells in primary cell culture are impermeable to horseradish peroxidase (HRP) after 5 min of incubation. However, following 1-5 min of exposure to 1.6 M arabinose, HRP penetrates the extracellular space between successive tight junctions. Endothelial cells in control cultures contain a small number of cytoplasmic pits and vesicles containing HRP which do not increase in number after hyperosmotic treatment.
KW - arabinose
KW - brain capillary endothelium
KW - horseradish peroxidase
KW - tight junctions
UR - http://www.scopus.com/inward/record.url?scp=0021187111&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021187111&partnerID=8YFLogxK
U2 - 10.1016/0006-8993(84)90254-3
DO - 10.1016/0006-8993(84)90254-3
M3 - Article
C2 - 6733518
AN - SCOPUS:0021187111
VL - 302
SP - 383
EP - 386
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 2
ER -