Hyperimmune hens as a novel source of anti-Cryptosporidium antibodies suitable for passive immune transfer.

V. A. Cama, C. R. Sterling

Research output: Contribution to journalArticle

Abstract

Leghorn hens were subcutaneously immunized with 25 micrograms of Cryptosporidium parvum oocyst emulsified in Freund's complete adjuvant. A booster dose was injected 5 weeks later. Anti-Cryptosporidium activities of yolks and sera measured by an enzyme-linked immunosorbent assay (ELISA), demonstrated high levels in both sera and egg yolks which persisted for at least 17 wk. Preparations from yolks with high, medium and low anti-Cryptosporidium ELISA activities were used in a neonatal mouse model to assess their biological activities. A significant parasite reduction (P less than or equal to 0.001) was found between the high and all other groups. Hyperimmune eggs could be used as a source for passive immunity in cryptosporidiosis.

Original languageEnglish (US)
JournalJournal of Protozoology
Volume38
Issue number6
StatePublished - Nov 1991
Externally publishedYes

Fingerprint

Cryptosporidium
Anti-Idiotypic Antibodies
Enzyme-Linked Immunosorbent Assay
Cryptosporidium parvum
Cryptosporidiosis
Egg Yolk
Oocysts
Freund's Adjuvant
Serum
Eggs
Immunity
Parasites

ASJC Scopus subject areas

  • Parasitology

Cite this

Hyperimmune hens as a novel source of anti-Cryptosporidium antibodies suitable for passive immune transfer. / Cama, V. A.; Sterling, C. R.

In: Journal of Protozoology, Vol. 38, No. 6, 11.1991.

Research output: Contribution to journalArticle

@article{dda8ddad34244898ac48b7748d1191f8,
title = "Hyperimmune hens as a novel source of anti-Cryptosporidium antibodies suitable for passive immune transfer.",
abstract = "Leghorn hens were subcutaneously immunized with 25 micrograms of Cryptosporidium parvum oocyst emulsified in Freund's complete adjuvant. A booster dose was injected 5 weeks later. Anti-Cryptosporidium activities of yolks and sera measured by an enzyme-linked immunosorbent assay (ELISA), demonstrated high levels in both sera and egg yolks which persisted for at least 17 wk. Preparations from yolks with high, medium and low anti-Cryptosporidium ELISA activities were used in a neonatal mouse model to assess their biological activities. A significant parasite reduction (P less than or equal to 0.001) was found between the high and all other groups. Hyperimmune eggs could be used as a source for passive immunity in cryptosporidiosis.",
author = "Cama, {V. A.} and Sterling, {C. R.}",
year = "1991",
month = "11",
language = "English (US)",
volume = "38",
journal = "Journal of Eukaryotic Microbiology",
issn = "1066-5234",
publisher = "Wiley-Blackwell",
number = "6",

}

TY - JOUR

T1 - Hyperimmune hens as a novel source of anti-Cryptosporidium antibodies suitable for passive immune transfer.

AU - Cama, V. A.

AU - Sterling, C. R.

PY - 1991/11

Y1 - 1991/11

N2 - Leghorn hens were subcutaneously immunized with 25 micrograms of Cryptosporidium parvum oocyst emulsified in Freund's complete adjuvant. A booster dose was injected 5 weeks later. Anti-Cryptosporidium activities of yolks and sera measured by an enzyme-linked immunosorbent assay (ELISA), demonstrated high levels in both sera and egg yolks which persisted for at least 17 wk. Preparations from yolks with high, medium and low anti-Cryptosporidium ELISA activities were used in a neonatal mouse model to assess their biological activities. A significant parasite reduction (P less than or equal to 0.001) was found between the high and all other groups. Hyperimmune eggs could be used as a source for passive immunity in cryptosporidiosis.

AB - Leghorn hens were subcutaneously immunized with 25 micrograms of Cryptosporidium parvum oocyst emulsified in Freund's complete adjuvant. A booster dose was injected 5 weeks later. Anti-Cryptosporidium activities of yolks and sera measured by an enzyme-linked immunosorbent assay (ELISA), demonstrated high levels in both sera and egg yolks which persisted for at least 17 wk. Preparations from yolks with high, medium and low anti-Cryptosporidium ELISA activities were used in a neonatal mouse model to assess their biological activities. A significant parasite reduction (P less than or equal to 0.001) was found between the high and all other groups. Hyperimmune eggs could be used as a source for passive immunity in cryptosporidiosis.

UR - http://www.scopus.com/inward/record.url?scp=0026247793&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026247793&partnerID=8YFLogxK

M3 - Article

C2 - 1818193

AN - SCOPUS:0026247793

VL - 38

JO - Journal of Eukaryotic Microbiology

JF - Journal of Eukaryotic Microbiology

SN - 1066-5234

IS - 6

ER -