Hyperbaric oxygen: A means of decreasing ischemic epiphyseal damage in a pediatric rabbit model

Denton D. Weiss, Douglas M. Stevens, Ji Geng Yan, Kenneth L. Lynch, Gary R. Warnock, Hani S. Matloub

Research output: Contribution to journalArticle

Abstract

The effect of hyperbaric oxygen on epiphyseal ischemia was evaluated using a pediatric rabbit model. Forty-five animals were compared in this study: 23 from a control pilot study and 22 hyperbaric exposed animals. In each animal the right distal femoral and proximal tibial epiphyses were isolated on a popliteal vascular pedicle. The left leg acted as the control. The growth difference between the rabbit's hindlimbs was the means of comparison throughout the groups established. Warm ischemia was induced by applying a vascular clamp to the right popliteal artery for 12 hours (20 animals) and 7 hours (17 animals). The remaining 8 animals underwent a sham operation without interruption of epiphyseal perfusion. On completion of the ischemic period hyperbaric oxygen therapy (HBOT) was performed on 12 12-hour (12h-HBOT) and 10 7-hour (7h-HBOT) animals at 2 atmospheres for 90 minutes twice per day for 4 postoperative days. The animals were killed on either postoperative day 14 or 90. Measurement of longitudinal bone growth was performed on the 90-day animals from serial radiographs at the time of surgery and then at 1 month, 2 months, and 3 months after surgery. There was no significant difference in longitudinal bone growth between the sham- operated and the 7h-HBOT animals at 1, 2, and 3 months. There was a statistically significant difference, however, between the normal growth of the 7h-HBOT group compared with the abnormal growth of the 7-hour, 12-hour, and 12h-HBOT animals. Histology was consistent, with the bone growth data demonstrating relative normalcy of the 7h-HBOT group epiphyseal plates versus severe architectural aberrance and necrosis of the 12h-HBOT group epiphyses. Our experimental data indicate that a clinical trial should be instituted using HBO for pediatric replantation patients when warm ischemia exceeds 7 hours. Copyright (C) 2000 by the American Society for Surgery of the Hand.

Original languageEnglish (US)
Pages (from-to)159-165
Number of pages7
JournalJournal of Hand Surgery
Volume25
Issue number1
DOIs
StatePublished - Jan 2000
Externally publishedYes

Fingerprint

Hyperbaric Oxygenation
Pediatrics
Oxygen
Rabbits
Bone Development
Warm Ischemia
Epiphyses
Blood Vessels
Growth
Animal Rights
Popliteal Artery
Growth Plate
Replantation
Hindlimb
Thigh
Atmosphere
Leg
Histology
Necrosis
Ischemia

Keywords

  • Hyperbaric oxygen
  • Ischemic epiphysis
  • Rabbit

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Surgery

Cite this

Weiss, D. D., Stevens, D. M., Yan, J. G., Lynch, K. L., Warnock, G. R., & Matloub, H. S. (2000). Hyperbaric oxygen: A means of decreasing ischemic epiphyseal damage in a pediatric rabbit model. Journal of Hand Surgery, 25(1), 159-165. https://doi.org/10.1053/jhsu.2000.jhsu025a0159

Hyperbaric oxygen : A means of decreasing ischemic epiphyseal damage in a pediatric rabbit model. / Weiss, Denton D.; Stevens, Douglas M.; Yan, Ji Geng; Lynch, Kenneth L.; Warnock, Gary R.; Matloub, Hani S.

In: Journal of Hand Surgery, Vol. 25, No. 1, 01.2000, p. 159-165.

Research output: Contribution to journalArticle

Weiss, DD, Stevens, DM, Yan, JG, Lynch, KL, Warnock, GR & Matloub, HS 2000, 'Hyperbaric oxygen: A means of decreasing ischemic epiphyseal damage in a pediatric rabbit model', Journal of Hand Surgery, vol. 25, no. 1, pp. 159-165. https://doi.org/10.1053/jhsu.2000.jhsu025a0159
Weiss, Denton D. ; Stevens, Douglas M. ; Yan, Ji Geng ; Lynch, Kenneth L. ; Warnock, Gary R. ; Matloub, Hani S. / Hyperbaric oxygen : A means of decreasing ischemic epiphyseal damage in a pediatric rabbit model. In: Journal of Hand Surgery. 2000 ; Vol. 25, No. 1. pp. 159-165.
@article{d102e5f2544046138b8ef5358d027ca5,
title = "Hyperbaric oxygen: A means of decreasing ischemic epiphyseal damage in a pediatric rabbit model",
abstract = "The effect of hyperbaric oxygen on epiphyseal ischemia was evaluated using a pediatric rabbit model. Forty-five animals were compared in this study: 23 from a control pilot study and 22 hyperbaric exposed animals. In each animal the right distal femoral and proximal tibial epiphyses were isolated on a popliteal vascular pedicle. The left leg acted as the control. The growth difference between the rabbit's hindlimbs was the means of comparison throughout the groups established. Warm ischemia was induced by applying a vascular clamp to the right popliteal artery for 12 hours (20 animals) and 7 hours (17 animals). The remaining 8 animals underwent a sham operation without interruption of epiphyseal perfusion. On completion of the ischemic period hyperbaric oxygen therapy (HBOT) was performed on 12 12-hour (12h-HBOT) and 10 7-hour (7h-HBOT) animals at 2 atmospheres for 90 minutes twice per day for 4 postoperative days. The animals were killed on either postoperative day 14 or 90. Measurement of longitudinal bone growth was performed on the 90-day animals from serial radiographs at the time of surgery and then at 1 month, 2 months, and 3 months after surgery. There was no significant difference in longitudinal bone growth between the sham- operated and the 7h-HBOT animals at 1, 2, and 3 months. There was a statistically significant difference, however, between the normal growth of the 7h-HBOT group compared with the abnormal growth of the 7-hour, 12-hour, and 12h-HBOT animals. Histology was consistent, with the bone growth data demonstrating relative normalcy of the 7h-HBOT group epiphyseal plates versus severe architectural aberrance and necrosis of the 12h-HBOT group epiphyses. Our experimental data indicate that a clinical trial should be instituted using HBO for pediatric replantation patients when warm ischemia exceeds 7 hours. Copyright (C) 2000 by the American Society for Surgery of the Hand.",
keywords = "Hyperbaric oxygen, Ischemic epiphysis, Rabbit",
author = "Weiss, {Denton D.} and Stevens, {Douglas M.} and Yan, {Ji Geng} and Lynch, {Kenneth L.} and Warnock, {Gary R.} and Matloub, {Hani S.}",
year = "2000",
month = "1",
doi = "10.1053/jhsu.2000.jhsu025a0159",
language = "English (US)",
volume = "25",
pages = "159--165",
journal = "The Hand",
issn = "1753-1934",
publisher = "SAGE Publications Ltd",
number = "1",

}

TY - JOUR

T1 - Hyperbaric oxygen

T2 - A means of decreasing ischemic epiphyseal damage in a pediatric rabbit model

AU - Weiss, Denton D.

AU - Stevens, Douglas M.

AU - Yan, Ji Geng

AU - Lynch, Kenneth L.

AU - Warnock, Gary R.

AU - Matloub, Hani S.

PY - 2000/1

Y1 - 2000/1

N2 - The effect of hyperbaric oxygen on epiphyseal ischemia was evaluated using a pediatric rabbit model. Forty-five animals were compared in this study: 23 from a control pilot study and 22 hyperbaric exposed animals. In each animal the right distal femoral and proximal tibial epiphyses were isolated on a popliteal vascular pedicle. The left leg acted as the control. The growth difference between the rabbit's hindlimbs was the means of comparison throughout the groups established. Warm ischemia was induced by applying a vascular clamp to the right popliteal artery for 12 hours (20 animals) and 7 hours (17 animals). The remaining 8 animals underwent a sham operation without interruption of epiphyseal perfusion. On completion of the ischemic period hyperbaric oxygen therapy (HBOT) was performed on 12 12-hour (12h-HBOT) and 10 7-hour (7h-HBOT) animals at 2 atmospheres for 90 minutes twice per day for 4 postoperative days. The animals were killed on either postoperative day 14 or 90. Measurement of longitudinal bone growth was performed on the 90-day animals from serial radiographs at the time of surgery and then at 1 month, 2 months, and 3 months after surgery. There was no significant difference in longitudinal bone growth between the sham- operated and the 7h-HBOT animals at 1, 2, and 3 months. There was a statistically significant difference, however, between the normal growth of the 7h-HBOT group compared with the abnormal growth of the 7-hour, 12-hour, and 12h-HBOT animals. Histology was consistent, with the bone growth data demonstrating relative normalcy of the 7h-HBOT group epiphyseal plates versus severe architectural aberrance and necrosis of the 12h-HBOT group epiphyses. Our experimental data indicate that a clinical trial should be instituted using HBO for pediatric replantation patients when warm ischemia exceeds 7 hours. Copyright (C) 2000 by the American Society for Surgery of the Hand.

AB - The effect of hyperbaric oxygen on epiphyseal ischemia was evaluated using a pediatric rabbit model. Forty-five animals were compared in this study: 23 from a control pilot study and 22 hyperbaric exposed animals. In each animal the right distal femoral and proximal tibial epiphyses were isolated on a popliteal vascular pedicle. The left leg acted as the control. The growth difference between the rabbit's hindlimbs was the means of comparison throughout the groups established. Warm ischemia was induced by applying a vascular clamp to the right popliteal artery for 12 hours (20 animals) and 7 hours (17 animals). The remaining 8 animals underwent a sham operation without interruption of epiphyseal perfusion. On completion of the ischemic period hyperbaric oxygen therapy (HBOT) was performed on 12 12-hour (12h-HBOT) and 10 7-hour (7h-HBOT) animals at 2 atmospheres for 90 minutes twice per day for 4 postoperative days. The animals were killed on either postoperative day 14 or 90. Measurement of longitudinal bone growth was performed on the 90-day animals from serial radiographs at the time of surgery and then at 1 month, 2 months, and 3 months after surgery. There was no significant difference in longitudinal bone growth between the sham- operated and the 7h-HBOT animals at 1, 2, and 3 months. There was a statistically significant difference, however, between the normal growth of the 7h-HBOT group compared with the abnormal growth of the 7-hour, 12-hour, and 12h-HBOT animals. Histology was consistent, with the bone growth data demonstrating relative normalcy of the 7h-HBOT group epiphyseal plates versus severe architectural aberrance and necrosis of the 12h-HBOT group epiphyses. Our experimental data indicate that a clinical trial should be instituted using HBO for pediatric replantation patients when warm ischemia exceeds 7 hours. Copyright (C) 2000 by the American Society for Surgery of the Hand.

KW - Hyperbaric oxygen

KW - Ischemic epiphysis

KW - Rabbit

UR - http://www.scopus.com/inward/record.url?scp=0033983183&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033983183&partnerID=8YFLogxK

U2 - 10.1053/jhsu.2000.jhsu025a0159

DO - 10.1053/jhsu.2000.jhsu025a0159

M3 - Article

C2 - 10642487

AN - SCOPUS:0033983183

VL - 25

SP - 159

EP - 165

JO - The Hand

JF - The Hand

SN - 1753-1934

IS - 1

ER -