Hyperactivity and altered mRNA isoform expression of the Cl-/HCO3 - anion-exchanger in the hypertrophied myocardium

Gladys Chiappe De Cingolani, Patricio Morgan, Cecilia Mundiña-Weilenmann, Joseph Casey, Jocelyne Fujinaga, María Camilión De Hurtado, Horacio Cingolani

Research output: Contribution to journalArticle

Abstract

Objective: The aim was to examine the regulation of the cardiac Na+-independent Cl-/HCO3 - exchanger (AE) mRNA isoform expression in association to the enhanced AE activity in the hypertrophied myocardium of spontaneously hypertensive rats (SHR). Methods: AE activity was determined by the initial rates of the pHi recovery from imposed intracellular alkalinization (forward mode of exchange) and the pHi rise induced by Cl- removal (reverse mode). Net HCO3 - (JHCO3 -) efflux and influx were respectively determined. AE mRNA isoforms were analyzed by Northern blot with specific probes to detect AE1, AE2 and AE3 mRNAs. Results: Initial JHCO3 - efflux after imposed alkaline load (pHi≅7.5) was higher in SHR than in normotensive WKY rats (3.01±0.33, n=7, vs. 0.64±0.29 mM/min, n=5, PHCO3 - influx induced by Cl- deprivation was also increased in SHR, 4.24±0.56 mM/min (n=10) versus 2.31±0.26 (n=10, P

Original languageEnglish (US)
Pages (from-to)71-79
Number of pages9
JournalCardiovascular Research
Volume51
Issue number1
DOIs
StatePublished - 2001
Externally publishedYes

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Chloride-Bicarbonate Antiporters
RNA Isoforms
Inbred SHR Rats
Anions
Myocardium
Inbred WKY Rats
Northern Blotting
Messenger RNA

Keywords

  • Gene expression
  • Hypertension
  • Hypertrophy
  • Ion exchangers
  • Membrane transport

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Chiappe De Cingolani, G., Morgan, P., Mundiña-Weilenmann, C., Casey, J., Fujinaga, J., Camilión De Hurtado, M., & Cingolani, H. (2001). Hyperactivity and altered mRNA isoform expression of the Cl-/HCO3 - anion-exchanger in the hypertrophied myocardium. Cardiovascular Research, 51(1), 71-79. https://doi.org/10.1016/S0008-6363(01)00276-0

Hyperactivity and altered mRNA isoform expression of the Cl-/HCO3 - anion-exchanger in the hypertrophied myocardium. / Chiappe De Cingolani, Gladys; Morgan, Patricio; Mundiña-Weilenmann, Cecilia; Casey, Joseph; Fujinaga, Jocelyne; Camilión De Hurtado, María; Cingolani, Horacio.

In: Cardiovascular Research, Vol. 51, No. 1, 2001, p. 71-79.

Research output: Contribution to journalArticle

Chiappe De Cingolani, G, Morgan, P, Mundiña-Weilenmann, C, Casey, J, Fujinaga, J, Camilión De Hurtado, M & Cingolani, H 2001, 'Hyperactivity and altered mRNA isoform expression of the Cl-/HCO3 - anion-exchanger in the hypertrophied myocardium', Cardiovascular Research, vol. 51, no. 1, pp. 71-79. https://doi.org/10.1016/S0008-6363(01)00276-0
Chiappe De Cingolani G, Morgan P, Mundiña-Weilenmann C, Casey J, Fujinaga J, Camilión De Hurtado M et al. Hyperactivity and altered mRNA isoform expression of the Cl-/HCO3 - anion-exchanger in the hypertrophied myocardium. Cardiovascular Research. 2001;51(1):71-79. https://doi.org/10.1016/S0008-6363(01)00276-0
Chiappe De Cingolani, Gladys ; Morgan, Patricio ; Mundiña-Weilenmann, Cecilia ; Casey, Joseph ; Fujinaga, Jocelyne ; Camilión De Hurtado, María ; Cingolani, Horacio. / Hyperactivity and altered mRNA isoform expression of the Cl-/HCO3 - anion-exchanger in the hypertrophied myocardium. In: Cardiovascular Research. 2001 ; Vol. 51, No. 1. pp. 71-79.
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AU - Mundiña-Weilenmann, Cecilia

AU - Casey, Joseph

AU - Fujinaga, Jocelyne

AU - Camilión De Hurtado, María

AU - Cingolani, Horacio

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