Hydrogen peroxide mediates reperfusion injury in the isolated rat heart

James M. Brown, Lance S. Terada, Michael A. Grosso, Glenn Whitman, Stephen E. Velasco, Anita Patt, Alden H. Harken, John E. Repine

Research output: Contribution to journalArticle

Abstract

In an isolated, normothermic rat heart model (Langendorff, 37 °C), dimethylthiourea (DMTU) infusion only during reperfusion reduced both injury and measurable hydrogen peroxide (H2O2) concentrations after global ischemia. Cardiac function was assessed by measurement of ventricular developed pressure (DP). H2O2 was assessed using H2O2 dependent aminotriazole inactivation of myocardial catalase. Depletion of xanthine oxidase by two methods (tungsten or allopurinol inhibition) also improved recovery of function and H2O2 production. The results indicate that XO derived H2O2 contributes to myocardial reperfusion injury.

Original languageEnglish (US)
Pages (from-to)173-175
Number of pages3
JournalMolecular and Cellular Biochemistry
Volume84
Issue number2
DOIs
StatePublished - Dec 1988
Externally publishedYes

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Keywords

  • allopurinol
  • eschemia-reperfusion
  • global ischemia
  • xanthine oxidase depletion

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Clinical Biochemistry
  • Cell Biology

Cite this

Brown, J. M., Terada, L. S., Grosso, M. A., Whitman, G., Velasco, S. E., Patt, A., Harken, A. H., & Repine, J. E. (1988). Hydrogen peroxide mediates reperfusion injury in the isolated rat heart. Molecular and Cellular Biochemistry, 84(2), 173-175. https://doi.org/10.1007/BF00421052