Hydrogen peroxide mediates reperfusion injury in the isolated rat heart

James M. Brown; Lance S. Terada; Michael A. Grosso; Glenn J. Whitman; Stephen E. Velasco; Anita Patt; Alden H. Harken; John E. Repine

doi:10.1007/BF00421052

Hydrogen peroxide mediates reperfusion injury in the isolated rat heart

James M. Brown, Lance S. Terada, Michael A. Grosso, Glenn J. Whitman, Stephen E. Velasco, Anita Patt, Alden H. Harken, John E. Repine

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

In an isolated, normothermic rat heart model (Langendorff, 37 °C), dimethylthiourea (DMTU) infusion only during reperfusion reduced both injury and measurable hydrogen peroxide (H₂O₂) concentrations after global ischemia. Cardiac function was assessed by measurement of ventricular developed pressure (DP). H₂O₂ was assessed using H₂O₂ dependent aminotriazole inactivation of myocardial catalase. Depletion of xanthine oxidase by two methods (tungsten or allopurinol inhibition) also improved recovery of function and H₂O₂ production. The results indicate that XO derived H₂O₂ contributes to myocardial reperfusion injury.

Original language	English (US)
Pages (from-to)	173-175
Number of pages	3
Journal	Molecular and Cellular Biochemistry
Volume	84
Issue number	2
DOIs	https://doi.org/10.1007/BF00421052
State	Published - Dec 1988
Externally published	Yes

Keywords

allopurinol
eschemia-reperfusion
global ischemia
xanthine oxidase depletion

ASJC Scopus subject areas

Molecular Biology
Clinical Biochemistry
Cell Biology

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10.1007/BF00421052

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title = "Hydrogen peroxide mediates reperfusion injury in the isolated rat heart",

abstract = "In an isolated, normothermic rat heart model (Langendorff, 37 °C), dimethylthiourea (DMTU) infusion only during reperfusion reduced both injury and measurable hydrogen peroxide (H2O2) concentrations after global ischemia. Cardiac function was assessed by measurement of ventricular developed pressure (DP). H2O2 was assessed using H2O2 dependent aminotriazole inactivation of myocardial catalase. Depletion of xanthine oxidase by two methods (tungsten or allopurinol inhibition) also improved recovery of function and H2O2 production. The results indicate that XO derived H2O2 contributes to myocardial reperfusion injury.",

keywords = "allopurinol, eschemia-reperfusion, global ischemia, xanthine oxidase depletion",

author = "Brown, {James M.} and Terada, {Lance S.} and Grosso, {Michael A.} and Whitman, {Glenn J.} and Velasco, {Stephen E.} and Anita Patt and Harken, {Alden H.} and Repine, {John E.}",

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language = "English (US)",

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T1 - Hydrogen peroxide mediates reperfusion injury in the isolated rat heart

AU - Brown, James M.

AU - Terada, Lance S.

AU - Grosso, Michael A.

AU - Whitman, Glenn J.

AU - Velasco, Stephen E.

AU - Patt, Anita

AU - Harken, Alden H.

AU - Repine, John E.

PY - 1988/12

Y1 - 1988/12

N2 - In an isolated, normothermic rat heart model (Langendorff, 37 °C), dimethylthiourea (DMTU) infusion only during reperfusion reduced both injury and measurable hydrogen peroxide (H2O2) concentrations after global ischemia. Cardiac function was assessed by measurement of ventricular developed pressure (DP). H2O2 was assessed using H2O2 dependent aminotriazole inactivation of myocardial catalase. Depletion of xanthine oxidase by two methods (tungsten or allopurinol inhibition) also improved recovery of function and H2O2 production. The results indicate that XO derived H2O2 contributes to myocardial reperfusion injury.

AB - In an isolated, normothermic rat heart model (Langendorff, 37 °C), dimethylthiourea (DMTU) infusion only during reperfusion reduced both injury and measurable hydrogen peroxide (H2O2) concentrations after global ischemia. Cardiac function was assessed by measurement of ventricular developed pressure (DP). H2O2 was assessed using H2O2 dependent aminotriazole inactivation of myocardial catalase. Depletion of xanthine oxidase by two methods (tungsten or allopurinol inhibition) also improved recovery of function and H2O2 production. The results indicate that XO derived H2O2 contributes to myocardial reperfusion injury.

KW - allopurinol

KW - eschemia-reperfusion

KW - global ischemia

KW - xanthine oxidase depletion

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Hydrogen peroxide mediates reperfusion injury in the isolated rat heart

Abstract

Keywords

ASJC Scopus subject areas

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