Hydrogels derived from central nervous system extracellular matrix

Christopher J. Medberry, Peter M. Crapo, Bernard F. Siu, Christopher A. Carruthers, Matthew T. Wolf, Shailesh P. Nagarkar, Vineet Agrawal, Kristen E. Jones, Jeremy Kelly, Scott A. Johnson, Sachin S. Velankar, Simon C. Watkins, Michel Modo, Stephen F. Badylak

Research output: Contribution to journalArticlepeer-review

Abstract

Biologic scaffolds composed of extracellular matrix (ECM) are commonly used repair devices in preclinical and clinical settings; however the use of these scaffolds for peripheral and central nervous system (CNS) repair has been limited. Biologic scaffolds developed from brain and spinal cord tissue have recently been described, yet the conformation of the harvested ECM limits therapeutic utility. An injectable CNS-ECM derived hydrogel capable of in vivo polymerization and conformation to irregular lesion geometries may aid in tissue reconstruction efforts following complex neurologic trauma. The objectives of the present study were to develop hydrogel forms of brain and spinal cord ECM and compare the resulting biochemical composition, mechanical properties, and neurotrophic potential of a brain derived cell line to a non-CNS-ECM hydrogel, urinary bladder matrix. Results showed distinct differences between compositions of brain ECM, spinal cord ECM, and urinary bladder matrix. The rheologic modulus of spinal cord ECM hydrogel was greater than that of brain ECM and urinary bladder matrix. All ECMs increased the number of cells expressing neurites, but only brain ECM increased neurite length, suggesting a possible tissue-specific effect. All hydrogels promoted three-dimensional uni- or bi-polar neurite outgrowth following 7 days in culture. These results suggest that CNS-ECM hydrogels may provide supportive scaffolding to promote in vivo axonal repair.

Original languageEnglish (US)
Pages (from-to)1033-1040
Number of pages8
JournalBiomaterials
Volume34
Issue number4
DOIs
StatePublished - Jan 2013

Keywords

  • Central nervous system
  • ECM (extracellular matrix)
  • Hydrogel
  • Regenerative medicine
  • Scaffold
  • Tissue engineering

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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