Abstract
Achieving stable, long-term performance of implanted neural prosthetic devices has been challenging because of implantation related neuron loss and a foreign body response that results in encapsulating glial scar formation. To improve neuron-prosthesis integration and form chronic, stable interfaces, we investigated the potential of neurotrophin-eluting hydrogel-electrospun fiber mat (EFM) composite coatings. In particular, poly(ethylene glycol)-poly(ε-caprolactone) (PEGPCL) hydrogel-poly(ε-caprolactone) EFM composites were applied as coatings for multielectrode arrays. Coatings were stable and persisted on electrode surfaces for over 1 month under an agarose gel tissue phantom and over 9 months in a PBS immersion bath. To demonstrate drug release, a neurotrophin, nerve growth factor (NGF), was loaded in the PEGPCL hydrogel layer, and coating cytotoxicity and sustained NGF release were evaluated using a PC12 cell culture model. Quantitative MTT assays showed that these coatings had no significant toxicity toward PC12 cells, and neurite extension at day 7 and 14 confirmed sustained release of NGF at biologically significant concentrations for at least 2 weeks. Our results demonstrate that hydrogel-EFM composite materials can be applied to neural prostheses to improve neuron-electrode proximity and enhance long-term device performance and function.
Original language | English (US) |
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Pages (from-to) | 1-8 |
Number of pages | 8 |
Journal | Frontiers in Neuroengineering |
Issue number | MARCH |
DOIs | |
State | Published - Mar 2011 |
Externally published | Yes |
Keywords
- Coatings
- Drug release
- Electrospun fibers
- Hydrogels
- Neural prostheses
ASJC Scopus subject areas
- Neuroscience (miscellaneous)
- Biophysics
- Biomedical Engineering