Hybrid selection of transcribed sequences from microdissected DNA: Isolation of genes within an amplified region at 20q11-q13.2 in breast cancer

Xin Yuan Guan, Jun Xu, Sarah L. Anzick, Hongen Zhang, Jeffrey M. Trent, Paul S. Meltzer

Research output: Contribution to journalArticle

Abstract

In human breast carcinomas, increased copy number of DNA sequences derived from the long arm of chromosome 20 (20q) has been commonly observed by both chromosome microdissection and comparative genomic hybridization. This chromosomal region is likely to contain one or more genes that are the biological targets of this amplification event. We describe here the utilization of a chromosome microdissection-hybrid selection strategy to isolate transcribed sequences from microdissected homogeneously staining regions encompassing 20q. Using this strategy, we have isolated three novel amplified genes (termed AIB1, AIB3, and AIB4) from a cDNA library constructed from the 20q amplified breast cancer cell line BT-474. These three genes were mapped to 20q11 (AIB3 and AIB4) and 20q12 (AIB1) by fluorescence in situ hybridization. Our results indicate an unsuspected complexity to the amplification pattern of 20q in breast cancer and provide probes that will be useful for further characterization of tumor specimens.

Original languageEnglish (US)
Pages (from-to)3446-3450
Number of pages5
JournalCancer Research
Volume56
Issue number15
StatePublished - Aug 1 1996
Externally publishedYes

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Microdissection
Breast Neoplasms
Chromosomes
Chromosomes, Human, Pair 20
Genes
Comparative Genomic Hybridization
Fluorescence In Situ Hybridization
Gene Library
Staining and Labeling
Cell Line
Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Guan, X. Y., Xu, J., Anzick, S. L., Zhang, H., Trent, J. M., & Meltzer, P. S. (1996). Hybrid selection of transcribed sequences from microdissected DNA: Isolation of genes within an amplified region at 20q11-q13.2 in breast cancer. Cancer Research, 56(15), 3446-3450.

Hybrid selection of transcribed sequences from microdissected DNA : Isolation of genes within an amplified region at 20q11-q13.2 in breast cancer. / Guan, Xin Yuan; Xu, Jun; Anzick, Sarah L.; Zhang, Hongen; Trent, Jeffrey M.; Meltzer, Paul S.

In: Cancer Research, Vol. 56, No. 15, 01.08.1996, p. 3446-3450.

Research output: Contribution to journalArticle

Guan, XY, Xu, J, Anzick, SL, Zhang, H, Trent, JM & Meltzer, PS 1996, 'Hybrid selection of transcribed sequences from microdissected DNA: Isolation of genes within an amplified region at 20q11-q13.2 in breast cancer', Cancer Research, vol. 56, no. 15, pp. 3446-3450.
Guan, Xin Yuan ; Xu, Jun ; Anzick, Sarah L. ; Zhang, Hongen ; Trent, Jeffrey M. ; Meltzer, Paul S. / Hybrid selection of transcribed sequences from microdissected DNA : Isolation of genes within an amplified region at 20q11-q13.2 in breast cancer. In: Cancer Research. 1996 ; Vol. 56, No. 15. pp. 3446-3450.
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AB - In human breast carcinomas, increased copy number of DNA sequences derived from the long arm of chromosome 20 (20q) has been commonly observed by both chromosome microdissection and comparative genomic hybridization. This chromosomal region is likely to contain one or more genes that are the biological targets of this amplification event. We describe here the utilization of a chromosome microdissection-hybrid selection strategy to isolate transcribed sequences from microdissected homogeneously staining regions encompassing 20q. Using this strategy, we have isolated three novel amplified genes (termed AIB1, AIB3, and AIB4) from a cDNA library constructed from the 20q amplified breast cancer cell line BT-474. These three genes were mapped to 20q11 (AIB3 and AIB4) and 20q12 (AIB1) by fluorescence in situ hybridization. Our results indicate an unsuspected complexity to the amplification pattern of 20q in breast cancer and provide probes that will be useful for further characterization of tumor specimens.

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