Human Yip1A specifies the localization of Yif1 to the Golgi apparatus

Changjiang Jin, Yang Zhang, Hongling Zhu, Kashif Ahmed, Chuanhai Fu, Xuebiao Yao

Research output: Contribution to journalArticle

Abstract

Yip1p and Yif1p are essential for transport from ER to Golgi stack during the early secretory pathway in budding yeast. Here, we report the identification and characterization of human Yif1. Sequence analysis revealed that human Yif1 (HsYif1), like most of the other YIP1 protein family members, contains multiple transmembrane segments. Double immunofluorescence study revealed co-distribution of HsYif1 with Golgi marker such as GS27. To delineate the function of HsYif1, we conducted a yeast two-hybrid assay and identified an interaction between human HsYif1 and HsYip1A, a homolog of yeast Yip1. In addition, our immunoprecipitation pull-down assay validates the interaction between HsYif1 and HsYip1A. Moreover, our immunofluorescence study demonstrates the co-distribution of HsYif1 and HsYip1A. Significantly, over-expression of mutant HsYip1A-lacked cytosolic region disrupts the localization of HsYif1 to the Golgi, suggesting that HsYip1A specifies the localization of HsYif1 to the Golgi. Therefore, we conclude that human Yip1A interacts with and determines the localization of HsYif1 to the Golgi apparatus.

Original languageEnglish (US)
Pages (from-to)16-22
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume334
Issue number1
DOIs
StatePublished - Aug 19 2005
Externally publishedYes

Fingerprint

Golgi Apparatus
Yeast
Fluorescent Antibody Technique
Assays
Forensic Anthropology
Two-Hybrid System Techniques
Saccharomycetales
Secretory Pathway
Immunoprecipitation
Sequence Analysis
Yeasts
Proteins

Keywords

  • ER
  • Golgi
  • Yif1
  • Yip1A

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Human Yip1A specifies the localization of Yif1 to the Golgi apparatus. / Jin, Changjiang; Zhang, Yang; Zhu, Hongling; Ahmed, Kashif; Fu, Chuanhai; Yao, Xuebiao.

In: Biochemical and Biophysical Research Communications, Vol. 334, No. 1, 19.08.2005, p. 16-22.

Research output: Contribution to journalArticle

Jin, Changjiang ; Zhang, Yang ; Zhu, Hongling ; Ahmed, Kashif ; Fu, Chuanhai ; Yao, Xuebiao. / Human Yip1A specifies the localization of Yif1 to the Golgi apparatus. In: Biochemical and Biophysical Research Communications. 2005 ; Vol. 334, No. 1. pp. 16-22.
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