Human viruses and viral indicators in marine water at two recreational beaches in Southern California, USA

David C. Love, Roberto A. Rodriguez, Christopher D. Gibbons, John F. Griffith, Qilu Yu, Jill R. Stewart, Mark D. Sobsey

Research output: Contribution to journalArticle

Abstract

Waterborne enteric viruses may pose disease risks to bather health but occurrence of these viruses has been difficult to characterize at recreational beaches. The aim of this study was to evaluate water for human virus occurrence at two Southern California recreational beaches with a history of beach closures. Human enteric viruses (adenovirus and norovirus) and viral indicators (F+ and somatic coliphages) were measured in water samples over a 4-month period from Avalon Beach, Catalina Island (n = 324) and Doheny Beach, Orange County (n = 112). Human viruses were concentrated from 40 L samples and detected by nested reverse transcriptase polymerase chain reaction (PCR). Detection frequencies at Doheny Beach were 25.5% (adenovirus) and 22.3% (norovirus), and at Avalon Beach were 9.3% (adenovirus) and 0.7% (norovirus). Positive associations between adenoviruses and fecal coliforms were observed at Doheny (p = 0.02) and Avalon (p = 0.01) Beaches. Human viruses were present at both beaches at higher frequencies than previously detected in the region, suggesting that the virus detection methods presented here may better measure potential health risks to bathers. These virus recovery, concentration, and molecular detection methods are advancing practices so that analysis of enteric viruses can become more effective and routine for recreational water quality monitoring.

Original languageEnglish (US)
Pages (from-to)136-150
Number of pages15
JournalJournal of Water and Health
Volume12
Issue number1
DOIs
StatePublished - 2014
Externally publishedYes

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Keywords

  • Adenovirus
  • Bathing water
  • Coliphage
  • Detection methods
  • Norovirus

ASJC Scopus subject areas

  • Water Science and Technology
  • Waste Management and Disposal
  • Public Health, Environmental and Occupational Health
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Love, D. C., Rodriguez, R. A., Gibbons, C. D., Griffith, J. F., Yu, Q., Stewart, J. R., & Sobsey, M. D. (2014). Human viruses and viral indicators in marine water at two recreational beaches in Southern California, USA. Journal of Water and Health, 12(1), 136-150. https://doi.org/10.2166/wh.2013.078