Human umbilical vessels and cultured umbilical vein endothelial and smooth muscle cells lack detectable protein and mRNA encoding estrogen receptors

Valerie L. Baker, Victor A. Chao, James T. Murai, Charles J. Zaloudek, Robert N. Taylor

Research output: Contribution to journalArticle

Abstract

OBJECTIVE: To identify and characterize estrogen receptors in human umbilical vascular tissues and in cultured cells derived from the human umbilical vein. METHODS: Human umbilical vein endothelial (HUVE) and human umbilical vein smooth muscle (HUVSM) cells were isolated. Immunohistochemical, radioligand binding, Western immunoblotting, and reverse transcription-polymerase chain reaction (RT-PCR) methods were used to detect estrogen receptors in vascular tissues and in cells derived from the umbilical cord. RESULTS: Estrogen receptor protein was not detected in either umbilical vessel tissue or in isolated HUVE or HUVSM cells. Messenger RNAs for the classic estrogen receptor (α) and estrogen receptor β isoforms also were undetectable by RT-PCR. CONCLUSION: These findings suggest that the effects of estradiol observed in this widely used vascular model are mediated by very low concentrations of receptors that evade standard methods of detection. Alternatively, this steroid may affect umbilical vascular cells through mechanisms that do not involve the classic genomic estrogen-receptor pathway.

Original languageEnglish (US)
Pages (from-to)316-324
Number of pages9
JournalJournal of the Society for Gynecologic Investigation
Volume4
Issue number6
DOIs
StatePublished - Nov 1 1997
Externally publishedYes

Keywords

  • Estradiol
  • Estrogen receptors α and β
  • Human umbilical vein endothelial cells
  • Human umbilical vein smooth muscle cells

ASJC Scopus subject areas

  • Obstetrics and Gynecology

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