TY - JOUR
T1 - Human umbilical cord blood progenitors
T2 - The potential of these hematopoietic cells to become neural
AU - Chen, Ning
AU - Hudson, Jennifer E.
AU - Walczak, Piotr
AU - Misiuta, Iwona
AU - Garbuzova-Davis, Svitlana
AU - Jiang, Lixian
AU - Sanchez-Ramos, Juan
AU - Sanberg, Paul R.
AU - Zigova, Tanja
AU - Willing, Alison E.
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2005/11
Y1 - 2005/11
N2 - The mononuclear fraction from human umbilical cord blood (HUCB) contains a significant number of stem/progenitor cells that in theory could become any cell in the body, including neurons. Taking into consideration that transdifferentiation would be a very rare event and also knowing that overlapping genetic programs for hematopoiesis and neuropoiesis exist, we undertook a characterization of the HUCB mononuclear fraction, including analysis of cellular subpopulations and their morphology, cell viability, proliferation, and expression of neural and hematopoietic antigens. Two cell populations were apparent-adherent and floating fractions. The adherent fraction was mainly lymphocytes (∼53%) expressing hematopoietic antigens. Upon replate, the floating population had many cells that expressed stem cell antigens. More of the cells in this subfraction expressed neural proteins. Neurotrophin receptors trkB and trkC were present in both cell fractions, although expression was higher in the floating fraction. Our initial characterization suggests that a subpopulation of cells exists within the HUCB mononuclear fraction that seems to have the potential to become neural cells, which could then be used in the development of cell-based therapies for brain injuries and diseases.
AB - The mononuclear fraction from human umbilical cord blood (HUCB) contains a significant number of stem/progenitor cells that in theory could become any cell in the body, including neurons. Taking into consideration that transdifferentiation would be a very rare event and also knowing that overlapping genetic programs for hematopoiesis and neuropoiesis exist, we undertook a characterization of the HUCB mononuclear fraction, including analysis of cellular subpopulations and their morphology, cell viability, proliferation, and expression of neural and hematopoietic antigens. Two cell populations were apparent-adherent and floating fractions. The adherent fraction was mainly lymphocytes (∼53%) expressing hematopoietic antigens. Upon replate, the floating population had many cells that expressed stem cell antigens. More of the cells in this subfraction expressed neural proteins. Neurotrophin receptors trkB and trkC were present in both cell fractions, although expression was higher in the floating fraction. Our initial characterization suggests that a subpopulation of cells exists within the HUCB mononuclear fraction that seems to have the potential to become neural cells, which could then be used in the development of cell-based therapies for brain injuries and diseases.
KW - Human
KW - Multipotential differentiation hematopoietic and neural antigens
KW - Neuropoiesis
KW - Stem/progenitor cell
KW - Umbilical cord blood
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U2 - 10.1634/stemcells.2004-0284
DO - 10.1634/stemcells.2004-0284
M3 - Article
C2 - 16081669
AN - SCOPUS:28444488536
VL - 23
SP - 1560
EP - 1570
JO - Stem Cells
JF - Stem Cells
SN - 1066-5099
IS - 10
ER -