Human telomerase RNA and telomerase activity in immortal cell lines and tumor tissues

Ariel A. Avilion, Mieczyslaw A. Piatyszek, Jyothi Gupta, Jerry W. Shay, Silvia Bacchetti, Carol W Greider

Research output: Contribution to journalArticle

Abstract

Telomerase activity has been detected in many human immortal cell lines and in tumor tissues, whereas it is generally absent from primary cell strains and from many tumor adjacent tissue samples. With the recently cloned human telomerase RNA (hTR), we used Northern analysis to follow the levels of hTR in primary, precrisis, and immortalized cells. It was surprising that the amount of hTR was high in cell strains that lacked telomerase activity, and the levels did not parallel the increases in telomerase activity, which accompanies immortalization. In addition, although the hTR levels were somewhat higher in tumor samples compared to nontumor tissues, the level of hTR in a variety of different human tumors did not predict the level of telomerase activity in the tumor. Thus, whereas hTR was detected in all samples that have telomerase activity, the presence of the RNA was not a good a predictor of the presence or amount of telomerase activity.

Original languageEnglish (US)
Pages (from-to)645-650
Number of pages6
JournalCancer Research
Volume56
Issue number3
StatePublished - Feb 1 1996
Externally publishedYes

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Telomerase
Tumor Cell Line
Neoplasms
telomerase RNA
RNA

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Avilion, A. A., Piatyszek, M. A., Gupta, J., Shay, J. W., Bacchetti, S., & Greider, C. W. (1996). Human telomerase RNA and telomerase activity in immortal cell lines and tumor tissues. Cancer Research, 56(3), 645-650.

Human telomerase RNA and telomerase activity in immortal cell lines and tumor tissues. / Avilion, Ariel A.; Piatyszek, Mieczyslaw A.; Gupta, Jyothi; Shay, Jerry W.; Bacchetti, Silvia; Greider, Carol W.

In: Cancer Research, Vol. 56, No. 3, 01.02.1996, p. 645-650.

Research output: Contribution to journalArticle

Avilion, AA, Piatyszek, MA, Gupta, J, Shay, JW, Bacchetti, S & Greider, CW 1996, 'Human telomerase RNA and telomerase activity in immortal cell lines and tumor tissues', Cancer Research, vol. 56, no. 3, pp. 645-650.
Avilion AA, Piatyszek MA, Gupta J, Shay JW, Bacchetti S, Greider CW. Human telomerase RNA and telomerase activity in immortal cell lines and tumor tissues. Cancer Research. 1996 Feb 1;56(3):645-650.
Avilion, Ariel A. ; Piatyszek, Mieczyslaw A. ; Gupta, Jyothi ; Shay, Jerry W. ; Bacchetti, Silvia ; Greider, Carol W. / Human telomerase RNA and telomerase activity in immortal cell lines and tumor tissues. In: Cancer Research. 1996 ; Vol. 56, No. 3. pp. 645-650.
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