Human stem-progenitor cells from neonatal cord blood have greater hematopoietic expansion capacity than those from mobilized adult blood

Vivek M. Tanavde, Matthew T. Malehorn, Rachata Lumkul, Zhigang Gao, John Wingard, Elizabeth S. Garrett, Curt I. Civin

Research output: Contribution to journalArticle

Abstract

Objective. In this study we compared the hematopoietic capacity of CD34 + cell preparations from neonatal cord blood (CB) vs adult mobilized peripheral blood (PBSC) before and after ex vivo culture. Methods. CD34 + cell preparations purified from CB or PBSC were cultured in serum-free medium containing FKT: FLT-3 ligand (FL), KIT ligand (KL), and thrombopoietin (TPO). Results. After 1-4 weeks ex vivo culture, CB CD34 + cell preparations had greatly increased numbers of total cells, CD34 + cells, and colony-forming cells (CFC). In contrast, ex vivo-cultured PBSC CD34 + cell preparations generated far less in vitro assessed hematopoietic capacity. Nonobese diabetic severe combined immunodeficient mouse (NOD/SCID) engrafting potential (SEP) was maintained in ex vivo-cultured CB CD34 + cell preparations, whereas ex vivo-cultured PBSC lost SEP. CB CD34 + cells continued to proliferate throughout 3 weeks ex vivo, whereas after 1 week, no additional cell divisions were detected in PBSC CD34 + cells. After 3 weeks in culture, the average CB CD34 + cell had divided more than 5 times, as compared to only 2 times for the average PBSC CD34 + cell. Conclusion. CB CD34 + cell preparations generated massively increased in vitro assessed hematopoietic capacity and maintained SEP during 1- to 4-week ex vivo cultures. In contrast, ex vivo-cultured PBSC CD34 + cell preparations generated far less in vitro assessed hematopoietic capacity and decreased SEP. The differences in the in vitro proliferative indices of membrane dye-labeled CD34 + cells from CB vs PBSC correlated with these functional differences.

Original languageEnglish (US)
Pages (from-to)816-823
Number of pages8
JournalExperimental Hematology
Volume30
Issue number7
DOIs
StatePublished - 2002

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Fetal Blood
Stem Cells
Blood Cells
Ligands
Thrombopoietin
Inbred NOD Mouse
SCID Mice
Serum-Free Culture Media
Cell Division
Coloring Agents
Cell Count
Membranes
In Vitro Techniques

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

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Human stem-progenitor cells from neonatal cord blood have greater hematopoietic expansion capacity than those from mobilized adult blood. / Tanavde, Vivek M.; Malehorn, Matthew T.; Lumkul, Rachata; Gao, Zhigang; Wingard, John; Garrett, Elizabeth S.; Civin, Curt I.

In: Experimental Hematology, Vol. 30, No. 7, 2002, p. 816-823.

Research output: Contribution to journalArticle

Tanavde, Vivek M. ; Malehorn, Matthew T. ; Lumkul, Rachata ; Gao, Zhigang ; Wingard, John ; Garrett, Elizabeth S. ; Civin, Curt I. / Human stem-progenitor cells from neonatal cord blood have greater hematopoietic expansion capacity than those from mobilized adult blood. In: Experimental Hematology. 2002 ; Vol. 30, No. 7. pp. 816-823.
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abstract = "Objective. In this study we compared the hematopoietic capacity of CD34 + cell preparations from neonatal cord blood (CB) vs adult mobilized peripheral blood (PBSC) before and after ex vivo culture. Methods. CD34 + cell preparations purified from CB or PBSC were cultured in serum-free medium containing FKT: FLT-3 ligand (FL), KIT ligand (KL), and thrombopoietin (TPO). Results. After 1-4 weeks ex vivo culture, CB CD34 + cell preparations had greatly increased numbers of total cells, CD34 + cells, and colony-forming cells (CFC). In contrast, ex vivo-cultured PBSC CD34 + cell preparations generated far less in vitro assessed hematopoietic capacity. Nonobese diabetic severe combined immunodeficient mouse (NOD/SCID) engrafting potential (SEP) was maintained in ex vivo-cultured CB CD34 + cell preparations, whereas ex vivo-cultured PBSC lost SEP. CB CD34 + cells continued to proliferate throughout 3 weeks ex vivo, whereas after 1 week, no additional cell divisions were detected in PBSC CD34 + cells. After 3 weeks in culture, the average CB CD34 + cell had divided more than 5 times, as compared to only 2 times for the average PBSC CD34 + cell. Conclusion. CB CD34 + cell preparations generated massively increased in vitro assessed hematopoietic capacity and maintained SEP during 1- to 4-week ex vivo cultures. In contrast, ex vivo-cultured PBSC CD34 + cell preparations generated far less in vitro assessed hematopoietic capacity and decreased SEP. The differences in the in vitro proliferative indices of membrane dye-labeled CD34 + cells from CB vs PBSC correlated with these functional differences.",
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T1 - Human stem-progenitor cells from neonatal cord blood have greater hematopoietic expansion capacity than those from mobilized adult blood

AU - Tanavde, Vivek M.

AU - Malehorn, Matthew T.

AU - Lumkul, Rachata

AU - Gao, Zhigang

AU - Wingard, John

AU - Garrett, Elizabeth S.

AU - Civin, Curt I.

PY - 2002

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N2 - Objective. In this study we compared the hematopoietic capacity of CD34 + cell preparations from neonatal cord blood (CB) vs adult mobilized peripheral blood (PBSC) before and after ex vivo culture. Methods. CD34 + cell preparations purified from CB or PBSC were cultured in serum-free medium containing FKT: FLT-3 ligand (FL), KIT ligand (KL), and thrombopoietin (TPO). Results. After 1-4 weeks ex vivo culture, CB CD34 + cell preparations had greatly increased numbers of total cells, CD34 + cells, and colony-forming cells (CFC). In contrast, ex vivo-cultured PBSC CD34 + cell preparations generated far less in vitro assessed hematopoietic capacity. Nonobese diabetic severe combined immunodeficient mouse (NOD/SCID) engrafting potential (SEP) was maintained in ex vivo-cultured CB CD34 + cell preparations, whereas ex vivo-cultured PBSC lost SEP. CB CD34 + cells continued to proliferate throughout 3 weeks ex vivo, whereas after 1 week, no additional cell divisions were detected in PBSC CD34 + cells. After 3 weeks in culture, the average CB CD34 + cell had divided more than 5 times, as compared to only 2 times for the average PBSC CD34 + cell. Conclusion. CB CD34 + cell preparations generated massively increased in vitro assessed hematopoietic capacity and maintained SEP during 1- to 4-week ex vivo cultures. In contrast, ex vivo-cultured PBSC CD34 + cell preparations generated far less in vitro assessed hematopoietic capacity and decreased SEP. The differences in the in vitro proliferative indices of membrane dye-labeled CD34 + cells from CB vs PBSC correlated with these functional differences.

AB - Objective. In this study we compared the hematopoietic capacity of CD34 + cell preparations from neonatal cord blood (CB) vs adult mobilized peripheral blood (PBSC) before and after ex vivo culture. Methods. CD34 + cell preparations purified from CB or PBSC were cultured in serum-free medium containing FKT: FLT-3 ligand (FL), KIT ligand (KL), and thrombopoietin (TPO). Results. After 1-4 weeks ex vivo culture, CB CD34 + cell preparations had greatly increased numbers of total cells, CD34 + cells, and colony-forming cells (CFC). In contrast, ex vivo-cultured PBSC CD34 + cell preparations generated far less in vitro assessed hematopoietic capacity. Nonobese diabetic severe combined immunodeficient mouse (NOD/SCID) engrafting potential (SEP) was maintained in ex vivo-cultured CB CD34 + cell preparations, whereas ex vivo-cultured PBSC lost SEP. CB CD34 + cells continued to proliferate throughout 3 weeks ex vivo, whereas after 1 week, no additional cell divisions were detected in PBSC CD34 + cells. After 3 weeks in culture, the average CB CD34 + cell had divided more than 5 times, as compared to only 2 times for the average PBSC CD34 + cell. Conclusion. CB CD34 + cell preparations generated massively increased in vitro assessed hematopoietic capacity and maintained SEP during 1- to 4-week ex vivo cultures. In contrast, ex vivo-cultured PBSC CD34 + cell preparations generated far less in vitro assessed hematopoietic capacity and decreased SEP. The differences in the in vitro proliferative indices of membrane dye-labeled CD34 + cells from CB vs PBSC correlated with these functional differences.

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