TY - JOUR
T1 - Human stem-progenitor cells from neonatal cord blood have greater hematopoietic expansion capacity than those from mobilized adult blood
AU - Tanavde, Vivek M.
AU - Malehorn, Matthew T.
AU - Lumkul, Rachata
AU - Gao, Zhigang
AU - Wingard, John
AU - Garrett, Elizabeth S.
AU - Civin, Curt I.
PY - 2002
Y1 - 2002
N2 - Objective. In this study we compared the hematopoietic capacity of CD34 + cell preparations from neonatal cord blood (CB) vs adult mobilized peripheral blood (PBSC) before and after ex vivo culture. Methods. CD34 + cell preparations purified from CB or PBSC were cultured in serum-free medium containing FKT: FLT-3 ligand (FL), KIT ligand (KL), and thrombopoietin (TPO). Results. After 1-4 weeks ex vivo culture, CB CD34 + cell preparations had greatly increased numbers of total cells, CD34 + cells, and colony-forming cells (CFC). In contrast, ex vivo-cultured PBSC CD34 + cell preparations generated far less in vitro assessed hematopoietic capacity. Nonobese diabetic severe combined immunodeficient mouse (NOD/SCID) engrafting potential (SEP) was maintained in ex vivo-cultured CB CD34 + cell preparations, whereas ex vivo-cultured PBSC lost SEP. CB CD34 + cells continued to proliferate throughout 3 weeks ex vivo, whereas after 1 week, no additional cell divisions were detected in PBSC CD34 + cells. After 3 weeks in culture, the average CB CD34 + cell had divided more than 5 times, as compared to only 2 times for the average PBSC CD34 + cell. Conclusion. CB CD34 + cell preparations generated massively increased in vitro assessed hematopoietic capacity and maintained SEP during 1- to 4-week ex vivo cultures. In contrast, ex vivo-cultured PBSC CD34 + cell preparations generated far less in vitro assessed hematopoietic capacity and decreased SEP. The differences in the in vitro proliferative indices of membrane dye-labeled CD34 + cells from CB vs PBSC correlated with these functional differences.
AB - Objective. In this study we compared the hematopoietic capacity of CD34 + cell preparations from neonatal cord blood (CB) vs adult mobilized peripheral blood (PBSC) before and after ex vivo culture. Methods. CD34 + cell preparations purified from CB or PBSC were cultured in serum-free medium containing FKT: FLT-3 ligand (FL), KIT ligand (KL), and thrombopoietin (TPO). Results. After 1-4 weeks ex vivo culture, CB CD34 + cell preparations had greatly increased numbers of total cells, CD34 + cells, and colony-forming cells (CFC). In contrast, ex vivo-cultured PBSC CD34 + cell preparations generated far less in vitro assessed hematopoietic capacity. Nonobese diabetic severe combined immunodeficient mouse (NOD/SCID) engrafting potential (SEP) was maintained in ex vivo-cultured CB CD34 + cell preparations, whereas ex vivo-cultured PBSC lost SEP. CB CD34 + cells continued to proliferate throughout 3 weeks ex vivo, whereas after 1 week, no additional cell divisions were detected in PBSC CD34 + cells. After 3 weeks in culture, the average CB CD34 + cell had divided more than 5 times, as compared to only 2 times for the average PBSC CD34 + cell. Conclusion. CB CD34 + cell preparations generated massively increased in vitro assessed hematopoietic capacity and maintained SEP during 1- to 4-week ex vivo cultures. In contrast, ex vivo-cultured PBSC CD34 + cell preparations generated far less in vitro assessed hematopoietic capacity and decreased SEP. The differences in the in vitro proliferative indices of membrane dye-labeled CD34 + cells from CB vs PBSC correlated with these functional differences.
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U2 - 10.1016/S0301-472X(02)00818-4
DO - 10.1016/S0301-472X(02)00818-4
M3 - Article
C2 - 12135681
AN - SCOPUS:0036328834
SN - 0301-472X
VL - 30
SP - 816
EP - 823
JO - Experimental Hematology
JF - Experimental Hematology
IS - 7
ER -