Human stem cell-derived spinal cord astrocytes with defined mature or reactive phenotypes

Laurent Roybon, NunoJ Lamas, Alejandro Garcia-Diaz, EunJu Yang, Rita Sattler, Vernice Jackson-Lewis, YoonA Kim, C. Alan Kachel, Jeffrey D Rothstein, Serge Przedborski, Hynek Wichterle, ChristopherE Henderson

Research output: Contribution to journalArticle

Abstract

Differentiation of astrocytes from human stem cells has significant potential for analysis of their role in normal brain function and disease, but existing protocols generate only immature astrocytes. Using early neuralization, we generated spinal cord astrocytes from mouse or human embryonic or induced pluripotent stem cells with high efficiency. Remarkably, short exposure to fibroblast growth factor 1 (FGF1) or FGF2 was sufficient to direct these astrocytes selectively toward a mature quiescent phenotype, as judged by both marker expression and functional analysis. In contrast, tumor necrosis factor alpha and interleukin-1β, but not FGFs, induced multiple elements of a reactive inflammatory phenotype but did not affect maturation. These phenotypically defined, scalable populations of spinal cord astrocytes will be important both for studying normal astrocyte function and for modeling human pathological processes invitro

Original languageEnglish (US)
Pages (from-to)1035-1048
Number of pages14
JournalCell Reports
Volume4
Issue number5
DOIs
StatePublished - May 12 2013

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Roybon, L., Lamas, N., Garcia-Diaz, A., Yang, E., Sattler, R., Jackson-Lewis, V., Kim, Y., Kachel, C. A., Rothstein, J. D., Przedborski, S., Wichterle, H., & Henderson, C. (2013). Human stem cell-derived spinal cord astrocytes with defined mature or reactive phenotypes. Cell Reports, 4(5), 1035-1048. https://doi.org/10.1016/j.celrep.2013.06.021