Human rad21 gene, hHR21sp, is downregulated by hypoxia in human tumor cells

Myoung Sook Kim, Jin Hyen Baek, Moon Kyoung Bae, Kyu Won Kim

Research output: Contribution to journalArticle

Abstract

To identify genes differentially expressed under normoxic (21% O2) or hypoxic (1% O2) conditions, we used the technique of mRNA differential display using total RNA extracted from Chang human liver cells. Among downregulated genes by hypoxia, we focused on hHR21SP (human homologue of rad21 S. pombe) that is involved in DNA double-strand break repair. Northern blot analysis revealed that mRNA expression of hHR21SP was inhibited by hypoxia in various tumor cell lines, such as HepG2, SKHep1, MCF7, and HT1080 cells. We also found that hypoglycemia and heat shock significantly decreased the hHR21SP level, indicating that a DNA double-strand break repair gene, hHR21SP might be regulated by environmental stresses. In addition, wortmannin, a DNA-dependent protein kinase (DNA-PK) inhibitor, decreased the level of hHR21SP mRNA, indicating that DNA-PK might be involved in the regulation of hHR21SP. These results propose a new understanding of hHR21SP regulations in human tumor cells.

Original languageEnglish (US)
Pages (from-to)1106-1112
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume281
Issue number5
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

DNA-Activated Protein Kinase
Schizosaccharomyces
Tumors
Down-Regulation
Genes
Cells
Messenger RNA
Repair
Neoplasms
DNA
Protein Kinase Inhibitors
Liver
Double-Stranded DNA Breaks
Display devices
RNA
Hypoxia
MCF-7 Cells
Gene Expression Profiling
Tumor Cell Line
Hypoglycemia

Keywords

  • Hypoxia
  • Rad21

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Human rad21 gene, hHR21sp, is downregulated by hypoxia in human tumor cells. / Sook Kim, Myoung; Hyen Baek, Jin; Bae, Moon Kyoung; Kim, Kyu Won.

In: Biochemical and Biophysical Research Communications, Vol. 281, No. 5, 2001, p. 1106-1112.

Research output: Contribution to journalArticle

Sook Kim, Myoung ; Hyen Baek, Jin ; Bae, Moon Kyoung ; Kim, Kyu Won. / Human rad21 gene, hHR21sp, is downregulated by hypoxia in human tumor cells. In: Biochemical and Biophysical Research Communications. 2001 ; Vol. 281, No. 5. pp. 1106-1112.
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