Human protamine-1 as an mri reporter gene based on chemical exchange

Amnon Bar-Shir, Guanshu Liu, Kannie W Y Chan, Nikita Oskolkov, Xiaolei Song, Nirbhay N. Yadav, Piotr Walczak, Michael T Mcmahon, Peter C Van Zijl, Jeff W Bulte, Assaf A. Gilad

Research output: Contribution to journalArticle

Abstract

Genetically engineered reporters have revolutionized the understanding of many biological processes. MRI-based reporter genes can dramatically improve our ability to monitor dynamic gene expression and allow coregistration of subcellular genetic information with high-resolution anatomical images. We have developed a biocompatible MRI reporter gene based on a human gene, the human protamine-1 (hPRM1). The arginine-rich hPRM1 (47% arginine residues) generates high MRI contrast based on the chemical exchange saturation transfer (CEST) contrast mechanism. The 51 amino acidlong hPRM1 protein was fully synthesized using microwave-assisted technology, and the CEST characteristics of this protein were compared to other CEST-based contrast agents. Both bacterial and human cells were engineered to express an optimized hPRM1 gene and showed higher CEST contrast compared to controls. Live cells expressing the hPRM1 reporter gene, and embedded in three-dimensional culture, also generated higher CEST contrast compared to wild-type live cells.

Original languageEnglish (US)
Pages (from-to)134-138
Number of pages5
JournalACS Chemical Biology
Volume9
Issue number1
DOIs
StatePublished - Jan 17 2014

Fingerprint

Protamines
Reporter Genes
Genes
Magnetic resonance imaging
Arginine
Cells
Biological Phenomena
Image resolution
Cell culture
Gene expression
Contrast Media
Microwaves
Technology
Gene Expression
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine

Cite this

Bar-Shir, A., Liu, G., Chan, K. W. Y., Oskolkov, N., Song, X., Yadav, N. N., ... Gilad, A. A. (2014). Human protamine-1 as an mri reporter gene based on chemical exchange. ACS Chemical Biology, 9(1), 134-138. https://doi.org/10.1021/cb400617q

Human protamine-1 as an mri reporter gene based on chemical exchange. / Bar-Shir, Amnon; Liu, Guanshu; Chan, Kannie W Y; Oskolkov, Nikita; Song, Xiaolei; Yadav, Nirbhay N.; Walczak, Piotr; Mcmahon, Michael T; Van Zijl, Peter C; Bulte, Jeff W; Gilad, Assaf A.

In: ACS Chemical Biology, Vol. 9, No. 1, 17.01.2014, p. 134-138.

Research output: Contribution to journalArticle

Bar-Shir A, Liu G, Chan KWY, Oskolkov N, Song X, Yadav NN et al. Human protamine-1 as an mri reporter gene based on chemical exchange. ACS Chemical Biology. 2014 Jan 17;9(1):134-138. https://doi.org/10.1021/cb400617q
Bar-Shir, Amnon ; Liu, Guanshu ; Chan, Kannie W Y ; Oskolkov, Nikita ; Song, Xiaolei ; Yadav, Nirbhay N. ; Walczak, Piotr ; Mcmahon, Michael T ; Van Zijl, Peter C ; Bulte, Jeff W ; Gilad, Assaf A. / Human protamine-1 as an mri reporter gene based on chemical exchange. In: ACS Chemical Biology. 2014 ; Vol. 9, No. 1. pp. 134-138.
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