Human prostatic acid phosphatases: III. counterimmunoelectrophoresis for rapid identification

Irene Mcdonald, N. R. Rose, E. J. Pontes, B. K. Choe

Research output: Contribution to journalArticlepeer-review

Abstract

When serial dilution of standard prostatic acid phosphatases (PAP) was reacted with constant amounts of anti-PAP serum by counterimmunoelectrophoresis (CIEP), the detection end point of enzyme concentration was 0.25 ng in a 10 μl sample volume. The PAP concentrations in unknowns can be quantitated by comparing the dilution end points of reference PAP with the testing samples. Serum PAP levels were determined by a radioimmunoassay (RIA) and CIEP using normal male and female sera and serum samples from patients with prostatic cancer and nonprostatic tumors. An excellent correlation was observed between the two assay results. According to RIA data, the concentration of PAP higher than 0.4 ng per 10 μl (or 4.0 ng per 100 μl) signify the elevation of serum or bone marrow PAP level beyond normal range (normal value 1.6 ± 0.8 ng/100 μl). Thus, the CIEP assay will be a simple and reliable screening method for the serum PAP levels in the clinical diagnosis of prostatic cancer.

Original languageEnglish (US)
Pages (from-to)235-239
Number of pages5
JournalSystems Biology in Reproductive Medicine
Volume1
Issue number3
DOIs
StatePublished - Jan 1 1978

ASJC Scopus subject areas

  • Reproductive Medicine
  • Urology

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