Human prostatic acid phosphatases. I. Isolation

B. K. Choe, E. J. Pontes, S. Bloink, N. R. Rose

Research output: Contribution to journalArticle

Abstract

A new purification procedure is described for the human prostatic acid phosphatase. The procedure included carboxy-methyl-Sephadex and Concanavalin A affinity column chromatography. The purified enzyme has a high specific enzyme activity and is free from any extraneous proteins judging from immunochemical criteria and biochemical criteria such as SDS-polyacrylamide gel electrophoresis. The purified enzyme produced a monospecific anti-PAP antisera in animals and this anti-PAP antibody did not cross-react with other human acid phosphatases.

Original languageEnglish (US)
Pages (from-to)221-226
Number of pages6
JournalArchives of Andrology
Volume1
Issue number3
StatePublished - 1978
Externally publishedYes

Fingerprint

Enzymes
Concanavalin A
Acid Phosphatase
Affinity Chromatography
Immune Sera
Polyacrylamide Gel Electrophoresis
Anti-Idiotypic Antibodies
prostatic acid phosphatase
Proteins
sephadex

ASJC Scopus subject areas

  • Endocrinology

Cite this

Choe, B. K., Pontes, E. J., Bloink, S., & Rose, N. R. (1978). Human prostatic acid phosphatases. I. Isolation. Archives of Andrology, 1(3), 221-226.

Human prostatic acid phosphatases. I. Isolation. / Choe, B. K.; Pontes, E. J.; Bloink, S.; Rose, N. R.

In: Archives of Andrology, Vol. 1, No. 3, 1978, p. 221-226.

Research output: Contribution to journalArticle

Choe, BK, Pontes, EJ, Bloink, S & Rose, NR 1978, 'Human prostatic acid phosphatases. I. Isolation', Archives of Andrology, vol. 1, no. 3, pp. 221-226.
Choe BK, Pontes EJ, Bloink S, Rose NR. Human prostatic acid phosphatases. I. Isolation. Archives of Andrology. 1978;1(3):221-226.
Choe, B. K. ; Pontes, E. J. ; Bloink, S. ; Rose, N. R. / Human prostatic acid phosphatases. I. Isolation. In: Archives of Andrology. 1978 ; Vol. 1, No. 3. pp. 221-226.
@article{daa54be6bdac43bd8ec028869726e329,
title = "Human prostatic acid phosphatases. I. Isolation",
abstract = "A new purification procedure is described for the human prostatic acid phosphatase. The procedure included carboxy-methyl-Sephadex and Concanavalin A affinity column chromatography. The purified enzyme has a high specific enzyme activity and is free from any extraneous proteins judging from immunochemical criteria and biochemical criteria such as SDS-polyacrylamide gel electrophoresis. The purified enzyme produced a monospecific anti-PAP antisera in animals and this anti-PAP antibody did not cross-react with other human acid phosphatases.",
author = "Choe, {B. K.} and Pontes, {E. J.} and S. Bloink and Rose, {N. R.}",
year = "1978",
language = "English (US)",
volume = "1",
pages = "221--226",
journal = "Systems Biology in Reproductive Medicine",
issn = "1939-6368",
publisher = "Informa Healthcare",
number = "3",

}

TY - JOUR

T1 - Human prostatic acid phosphatases. I. Isolation

AU - Choe, B. K.

AU - Pontes, E. J.

AU - Bloink, S.

AU - Rose, N. R.

PY - 1978

Y1 - 1978

N2 - A new purification procedure is described for the human prostatic acid phosphatase. The procedure included carboxy-methyl-Sephadex and Concanavalin A affinity column chromatography. The purified enzyme has a high specific enzyme activity and is free from any extraneous proteins judging from immunochemical criteria and biochemical criteria such as SDS-polyacrylamide gel electrophoresis. The purified enzyme produced a monospecific anti-PAP antisera in animals and this anti-PAP antibody did not cross-react with other human acid phosphatases.

AB - A new purification procedure is described for the human prostatic acid phosphatase. The procedure included carboxy-methyl-Sephadex and Concanavalin A affinity column chromatography. The purified enzyme has a high specific enzyme activity and is free from any extraneous proteins judging from immunochemical criteria and biochemical criteria such as SDS-polyacrylamide gel electrophoresis. The purified enzyme produced a monospecific anti-PAP antisera in animals and this anti-PAP antibody did not cross-react with other human acid phosphatases.

UR - http://www.scopus.com/inward/record.url?scp=0018226923&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018226923&partnerID=8YFLogxK

M3 - Article

VL - 1

SP - 221

EP - 226

JO - Systems Biology in Reproductive Medicine

JF - Systems Biology in Reproductive Medicine

SN - 1939-6368

IS - 3

ER -