Human prostatic acid phosphatases. I. Isolation

B. K. Choe, E. J. Pontes, S. Bloink, N. R. Rose

Research output: Contribution to journalArticle

Abstract

A new purification procedure is described for the human prostatic acid phosphatase. The procedure included carboxy-methyl-Sephadex and Concanavalin A affinity column chromatography. The purified enzyme has a high specific enzyme activity and is free from any extraneous proteins judging from immunochemical criteria and biochemical criteria such as SDS-polyacrylamide gel electrophoresis. The purified enzyme produced a monospecific anti-PAP antisera in animals and this anti-PAP antibody did not cross-react with other human acid phosphatases.

Original languageEnglish (US)
Pages (from-to)221-226
Number of pages6
JournalArchives of Andrology
Volume1
Issue number3
StatePublished - 1978
Externally publishedYes

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ASJC Scopus subject areas

  • Endocrinology

Cite this

Choe, B. K., Pontes, E. J., Bloink, S., & Rose, N. R. (1978). Human prostatic acid phosphatases. I. Isolation. Archives of Andrology, 1(3), 221-226.