Abstract
A new purification procedure is described for the human prostatic acid phosphatase. The procedure included carboxy-methyl-Sephadex and Concanavalin A affinity column chromatography. The purified enzyme has a high specific enzyme activity and is free from any extraneous proteins judging from immunochemical criteria and biochemical criteria such as SDS-polyacrylamide gel electrophoresis. The purified enzyme produced a monospecific anti-PAP antisera in animals and this anti-PAP antibody did not cross-react with other human acid phosphatases.
Original language | English (US) |
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Pages (from-to) | 221-226 |
Number of pages | 6 |
Journal | Archives of Andrology |
Volume | 1 |
Issue number | 3 |
State | Published - 1978 |
Externally published | Yes |
ASJC Scopus subject areas
- Endocrinology