TY - JOUR
T1 - Human primary CD4+ T cells activated in the presence of IFN-α2b express functional indoleamine 2, 3-dioxygenase
AU - Curreli, Sabrina
AU - Romerio, Fabio
AU - Mirandola, Prisco
AU - Barion, Paola
AU - Bemis, Kristi
AU - Zella, Davide
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 2001
Y1 - 2001
N2 - Indoleamine 2, 3-dioxygenase (IDO) is the rate-limiting enzyme in the catabolism of tryptophan. By creating a local microenvironment in which levels of tryptophan are low, IDO-expressing antigen-presenting cells (APC) could regulate T cell activation. This may be relevant to control both viral and bacterial replication as well as neoplastic cell growth. Interferon-α (IFN-α) is an antiviral cytokine affecting cellular differentiation. In addition, it reduces proliferation of CD4+ T cells by several molecular mechanisms. To dissect the molecular steps responsible for the INF-mediated antiproliferative activity, we sought to determine whether activated primary CD4+ T cells in the presence of IFN-α would produce IDO. We demonstrate here that IDO mRNA is not present in resting CD4+ T cells. Stimulation with anti-CD3 plus interleukin-2 (IL-2) induces expression of IDO mRNA (about 2000 copies/150, 000 cells), as determined by semiquantitative RT-PCR. When cells were stimulated in the presence of IFN-α, expression of IDO mRNA was significantly increased (more than 12, 000 copies/150, 000 cells). Functional analysis of IDO activity paralleled the results obtained with RT-PCR, demonstrating increased production of active enzyme in CD4+ T cells stimulated in the presence of IFN-α. Our results indicate that IFN-α modulates levels of IDO produced by activated CD4+ T cells. This would likely affect bystander cells by modifying levels of tryptophan in the local microenvironment.
AB - Indoleamine 2, 3-dioxygenase (IDO) is the rate-limiting enzyme in the catabolism of tryptophan. By creating a local microenvironment in which levels of tryptophan are low, IDO-expressing antigen-presenting cells (APC) could regulate T cell activation. This may be relevant to control both viral and bacterial replication as well as neoplastic cell growth. Interferon-α (IFN-α) is an antiviral cytokine affecting cellular differentiation. In addition, it reduces proliferation of CD4+ T cells by several molecular mechanisms. To dissect the molecular steps responsible for the INF-mediated antiproliferative activity, we sought to determine whether activated primary CD4+ T cells in the presence of IFN-α would produce IDO. We demonstrate here that IDO mRNA is not present in resting CD4+ T cells. Stimulation with anti-CD3 plus interleukin-2 (IL-2) induces expression of IDO mRNA (about 2000 copies/150, 000 cells), as determined by semiquantitative RT-PCR. When cells were stimulated in the presence of IFN-α, expression of IDO mRNA was significantly increased (more than 12, 000 copies/150, 000 cells). Functional analysis of IDO activity paralleled the results obtained with RT-PCR, demonstrating increased production of active enzyme in CD4+ T cells stimulated in the presence of IFN-α. Our results indicate that IFN-α modulates levels of IDO produced by activated CD4+ T cells. This would likely affect bystander cells by modifying levels of tryptophan in the local microenvironment.
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U2 - 10.1089/107999001750277916
DO - 10.1089/107999001750277916
M3 - Article
C2 - 11440641
AN - SCOPUS:0034922292
SN - 1079-9907
VL - 21
SP - 431
EP - 437
JO - Journal of Interferon and Cytokine Research
JF - Journal of Interferon and Cytokine Research
IS - 6
ER -