Human platelets possess multiple [3H]imipramine binding sites

John R. Ieni, Stephen R. Zukin, Herman M. Van Praag

Research output: Contribution to journalArticle

Abstract

Scatchard analysis of [3H]imipramine binding to human platelets over the concentration range of 0.1-250 nM revealed a biphasic concave upward curve. Computer-assisted analysis indicated the best fit of the data by a two-site model with apparent Kd values of 0.68 and 293 nM and apparent Bmax values of 802 fmol/mg protein and 12.72 pmol/mg protein for the high- and low-affinity components, respectively. These results demonstrate the complex manner in which [3H]imipramine binding to human platelets, suggesting a reason for inconsistencies in Bmax values reported in the literature. Further studies of these low- as well as high-affinity sites may help elucidate the functional role of [3H]imipramine binding sites in affective disorders.

Original languageEnglish (US)
Pages (from-to)669-672
Number of pages4
JournalEuropean Journal of Pharmacology
Volume106
Issue number3
DOIs
StatePublished - Nov 27 1984
Externally publishedYes

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Keywords

  • Depression
  • High affinity
  • Human platelets
  • Low affinity
  • [H]Imipramine binding

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

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