Human papillomavirus is a necessary cause of invasive cervical cancer worldwide

Jan M M Walboomers, Marcel V. Jacobs, M. Michele Manos, F. Xavier Bosch, J. Alain Kummer, Keerti V. Shah, Peter J F Snijders, Julian Peto, Chris J L M Meijer, Nubia Muñoz

Research output: Contribution to journalArticle

Abstract

A recent report that 93 per cent of invasive cervical cancers worldwide contain human papillomavirus (HPV) may be an underestimate, due to sample inadequacy or integration events affecting the HPV L1 gene, which is the target of the polymerase chain reaction (PCR)-based test which was used. The formerly HPV-negative cases from this study have therefore been reanalysed for HPV serum antibodies and HPV DNA. Serology for HPV 16 VLPs, E6, and E7 antibodies was performed on 49 of the 66 cases which were HPV-negative and a sample of 48 of the 866 cases which were HPV-positive in the original study. Moreover, 55 of the 66 formerly HPV-negative biopsies were also reanalysed by a sandwich procedure in which the outer sections in a series of sections are used for histological review, while the inner sections are assayed by three different HPV PCR assays targeting different open reading frames (ORFs). No significant difference was found in serology for HPV 16 proteins between the cases that were originally HPV PCR-negative and -positive. Type-specific E7 PCR for 14 high-risk HPV types detected HPV DNA in 38 (69 per cent) of the 55 originally HPV-negative and amplifiable specimens. The HPV types detected were 16, 18, 31, 33, 39, 45, 52, and 58. Two (4 per cent) additional cases were only HPV DNA-positive by E1 and/or L1 consensus PCR. Histological analysis of the 55 specimens revealed that 21 were qualitatively inadequate. Only two of the 34 adequate samples were HPV-negative on all PCR tests, as against 13 of the 21 that were inadequate (p

LanguageEnglish (US)
Pages12-19
Number of pages8
JournalJournal of Pathology
Volume189
Issue number1
DOIs
StatePublished - 1999

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Uterine Cervical Neoplasms
Polymerase Chain Reaction
Human papillomavirus 16
Serology
DNA
Antibodies
Open Reading Frames

Keywords

  • Cervical carcinoma
  • Epidemiology
  • Histology
  • HPV PCR
  • HPV serology

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Walboomers, J. M. M., Jacobs, M. V., Manos, M. M., Bosch, F. X., Kummer, J. A., Shah, K. V., ... Muñoz, N. (1999). Human papillomavirus is a necessary cause of invasive cervical cancer worldwide. Journal of Pathology, 189(1), 12-19. https://doi.org/10.1002/(SICI)1096-9896(199909)189:1<12::AID-PATH431>3.0.CO;2-F

Human papillomavirus is a necessary cause of invasive cervical cancer worldwide. / Walboomers, Jan M M; Jacobs, Marcel V.; Manos, M. Michele; Bosch, F. Xavier; Kummer, J. Alain; Shah, Keerti V.; Snijders, Peter J F; Peto, Julian; Meijer, Chris J L M; Muñoz, Nubia.

In: Journal of Pathology, Vol. 189, No. 1, 1999, p. 12-19.

Research output: Contribution to journalArticle

Walboomers, JMM, Jacobs, MV, Manos, MM, Bosch, FX, Kummer, JA, Shah, KV, Snijders, PJF, Peto, J, Meijer, CJLM & Muñoz, N 1999, 'Human papillomavirus is a necessary cause of invasive cervical cancer worldwide' Journal of Pathology, vol. 189, no. 1, pp. 12-19. https://doi.org/10.1002/(SICI)1096-9896(199909)189:1<12::AID-PATH431>3.0.CO;2-F
Walboomers, Jan M M ; Jacobs, Marcel V. ; Manos, M. Michele ; Bosch, F. Xavier ; Kummer, J. Alain ; Shah, Keerti V. ; Snijders, Peter J F ; Peto, Julian ; Meijer, Chris J L M ; Muñoz, Nubia. / Human papillomavirus is a necessary cause of invasive cervical cancer worldwide. In: Journal of Pathology. 1999 ; Vol. 189, No. 1. pp. 12-19.
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