Human papillomavirus infection and immunohistochemical p16INK4a expression as predictors of outcome in penile squamous cell carcinomas

Stephania M. Bezerra, Alcides Chaux, Mark W. Ball, Sheila F. Faraj, Enrico Munari, Nilda Gonzalez-Roibon, Rajni Sharma, Trinity J. Bivalacqua, Arthur L. Burnett, George J. Netto

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Summary Approximately 50% of penile squamous cell carcinomas (SCC) are associated with high-risk human papillomavirus (HR-HPV) infection. We evaluated the correlation of p16INK4a expression and HR-HPV with clinicopathological features and outcome in a cohort of patients with penile SCC. Two tissue microarrays were constructed from 53 invasive penile SCC at our hospital. p16INK4a expression was assessed by immunohistochemistry (CINtec Kit). High-risk human papillomavirus status was assessed by in situ hybridization (INFORM HPV III family 16 probe B cocktail). High-risk human papillomavirus was detected in 8 cases (15%), and p16INK4a overexpression was found in 23 cases (44%). Both markers showed a significant association with histologic subtype (P =.017 and P =.01, respectively) and lymphovascular invasion (P =.015 and P =.015, respectively). Regarding outcome analyses, neither HPV infection nor p16INK4a overexpression significantly predicted overall survival or cancer-specific survival using Cox proportional hazards regression model. High-risk human papillomavirus positivity and p16INK4a overexpression were significantly associated with histologic subtype and presence of lymphovascular invasion. Human papillomavirus status was not predictive of outcome in our cohort.

Original languageEnglish (US)
Pages (from-to)532-540
Number of pages9
JournalHuman pathology
Volume46
Issue number4
DOIs
StatePublished - Apr 1 2015

Keywords

  • HPV
  • Immunohistochemistry
  • In situ hybridization
  • Penile cancer
  • Squamous cell carcinoma
  • p16
  • p16

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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