Human papillomavirus (HPV) status of non-tobacco related squamous cell carcinomas of the lateral tongue

J. S. Poling, X. J. Ma, S. Bui, Y. Luo, R. Li, Wayne Martin Koch, W. H. Westra

Research output: Contribution to journalArticle

Abstract

Objectives The human papillomavirus (HPV) is an important cause of some head and neck squamous cell carcinomas (HNSCCs), but its role in cancer of the lateral tongue is debatable. Suspicion of HPV causation is heightened when these lateral tongue carcinomas arise in patients that are young and/or have never smoked. The purpose of this study was to determine the incidence of transcriptionally active high risk HPV in these tumors, with a particular emphasis on non-smoking patients who are often presumed to have HPV-positive tumors. Methods We evaluated 78 HNSCCs of the lateral tongue for the presence of HPV using p16 immunohistochemistry and an RNA in situ hybridization assay targeting HPV E6/E7 mRNA. The study population was enriched for patients without traditional risk factors such as smoking and drinking. Results P16 overexpression was detected in 9 (11.5%) of 78 cases, but HPV E6/E7 mRNA transcripts were detected in only 1 (1.3%) case (positive predictive value of p16 staining for the presence of transcriptionally active HPV = 0.12). HPV mRNA transcripts were not detected in any patient under 40 (n = 11), or in patients who had never smoked (n = 44), had quit smoking (n = 15), and/or were only light consumers of alcohol (n = 57). Conclusions HPV is not detected in the vast majority of lateral tongue carcinomas. In light of the observation that HPV plays little if any role in the development of these cancers, routine HPV testing is unwarranted, even for patients without traditional risk factors. P16 staining is not a reliable marker for the presence of transcriptionally active HPV at this particular anatomic site.

Original languageEnglish (US)
Pages (from-to)306-310
Number of pages5
JournalOral Oncology
Volume50
Issue number4
DOIs
StatePublished - 2014

Fingerprint

Tongue
Squamous Cell Carcinoma
Messenger RNA
Smoking
Tongue Neoplasms
Staining and Labeling
Carcinoma
Neoplasms
Causality
Drinking
In Situ Hybridization
Immunohistochemistry
Alcohols
RNA
Light

Keywords

  • Head and neck cancer
  • Oral cancer
  • P16
  • RNA in situ hybridization
  • Smoking
  • Tobacco

ASJC Scopus subject areas

  • Oncology
  • Oral Surgery
  • Cancer Research

Cite this

Human papillomavirus (HPV) status of non-tobacco related squamous cell carcinomas of the lateral tongue. / Poling, J. S.; Ma, X. J.; Bui, S.; Luo, Y.; Li, R.; Koch, Wayne Martin; Westra, W. H.

In: Oral Oncology, Vol. 50, No. 4, 2014, p. 306-310.

Research output: Contribution to journalArticle

Poling, J. S. ; Ma, X. J. ; Bui, S. ; Luo, Y. ; Li, R. ; Koch, Wayne Martin ; Westra, W. H. / Human papillomavirus (HPV) status of non-tobacco related squamous cell carcinomas of the lateral tongue. In: Oral Oncology. 2014 ; Vol. 50, No. 4. pp. 306-310.
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abstract = "Objectives The human papillomavirus (HPV) is an important cause of some head and neck squamous cell carcinomas (HNSCCs), but its role in cancer of the lateral tongue is debatable. Suspicion of HPV causation is heightened when these lateral tongue carcinomas arise in patients that are young and/or have never smoked. The purpose of this study was to determine the incidence of transcriptionally active high risk HPV in these tumors, with a particular emphasis on non-smoking patients who are often presumed to have HPV-positive tumors. Methods We evaluated 78 HNSCCs of the lateral tongue for the presence of HPV using p16 immunohistochemistry and an RNA in situ hybridization assay targeting HPV E6/E7 mRNA. The study population was enriched for patients without traditional risk factors such as smoking and drinking. Results P16 overexpression was detected in 9 (11.5{\%}) of 78 cases, but HPV E6/E7 mRNA transcripts were detected in only 1 (1.3{\%}) case (positive predictive value of p16 staining for the presence of transcriptionally active HPV = 0.12). HPV mRNA transcripts were not detected in any patient under 40 (n = 11), or in patients who had never smoked (n = 44), had quit smoking (n = 15), and/or were only light consumers of alcohol (n = 57). Conclusions HPV is not detected in the vast majority of lateral tongue carcinomas. In light of the observation that HPV plays little if any role in the development of these cancers, routine HPV testing is unwarranted, even for patients without traditional risk factors. P16 staining is not a reliable marker for the presence of transcriptionally active HPV at this particular anatomic site.",
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AU - Poling, J. S.

AU - Ma, X. J.

AU - Bui, S.

AU - Luo, Y.

AU - Li, R.

AU - Koch, Wayne Martin

AU - Westra, W. H.

PY - 2014

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N2 - Objectives The human papillomavirus (HPV) is an important cause of some head and neck squamous cell carcinomas (HNSCCs), but its role in cancer of the lateral tongue is debatable. Suspicion of HPV causation is heightened when these lateral tongue carcinomas arise in patients that are young and/or have never smoked. The purpose of this study was to determine the incidence of transcriptionally active high risk HPV in these tumors, with a particular emphasis on non-smoking patients who are often presumed to have HPV-positive tumors. Methods We evaluated 78 HNSCCs of the lateral tongue for the presence of HPV using p16 immunohistochemistry and an RNA in situ hybridization assay targeting HPV E6/E7 mRNA. The study population was enriched for patients without traditional risk factors such as smoking and drinking. Results P16 overexpression was detected in 9 (11.5%) of 78 cases, but HPV E6/E7 mRNA transcripts were detected in only 1 (1.3%) case (positive predictive value of p16 staining for the presence of transcriptionally active HPV = 0.12). HPV mRNA transcripts were not detected in any patient under 40 (n = 11), or in patients who had never smoked (n = 44), had quit smoking (n = 15), and/or were only light consumers of alcohol (n = 57). Conclusions HPV is not detected in the vast majority of lateral tongue carcinomas. In light of the observation that HPV plays little if any role in the development of these cancers, routine HPV testing is unwarranted, even for patients without traditional risk factors. P16 staining is not a reliable marker for the presence of transcriptionally active HPV at this particular anatomic site.

AB - Objectives The human papillomavirus (HPV) is an important cause of some head and neck squamous cell carcinomas (HNSCCs), but its role in cancer of the lateral tongue is debatable. Suspicion of HPV causation is heightened when these lateral tongue carcinomas arise in patients that are young and/or have never smoked. The purpose of this study was to determine the incidence of transcriptionally active high risk HPV in these tumors, with a particular emphasis on non-smoking patients who are often presumed to have HPV-positive tumors. Methods We evaluated 78 HNSCCs of the lateral tongue for the presence of HPV using p16 immunohistochemistry and an RNA in situ hybridization assay targeting HPV E6/E7 mRNA. The study population was enriched for patients without traditional risk factors such as smoking and drinking. Results P16 overexpression was detected in 9 (11.5%) of 78 cases, but HPV E6/E7 mRNA transcripts were detected in only 1 (1.3%) case (positive predictive value of p16 staining for the presence of transcriptionally active HPV = 0.12). HPV mRNA transcripts were not detected in any patient under 40 (n = 11), or in patients who had never smoked (n = 44), had quit smoking (n = 15), and/or were only light consumers of alcohol (n = 57). Conclusions HPV is not detected in the vast majority of lateral tongue carcinomas. In light of the observation that HPV plays little if any role in the development of these cancers, routine HPV testing is unwarranted, even for patients without traditional risk factors. P16 staining is not a reliable marker for the presence of transcriptionally active HPV at this particular anatomic site.

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