Human osteosarcoma cell lines are dependent on insulin-like growth factor I for in vitro growth

Cynthia Chavez Kappel, Maria C. Velez-Yanguas, Steven Hirschfeld, Lee J. Helman

Research output: Contribution to journalArticle

Abstract

Osteogenic sarcoma is the most common bone tumor of childhood and typically occurs during the adolescent growth spurt when growth hormone and insulin-like growth factor I (IGF-I) may be at their lifetime highest levels. Since IGF-I is involved in normal bone growth and differentiation, we have evaluated the possible role of IGF-I signaling in the growth of human osteogenic sarcoma cell lines. In this study, we demonstrate that in vitro survival of cells is dependent on exogenously supplied IGF-I. Furthermore, we show that these cells display functional IGF-I receptors on their surface and that in vitro growth is inhibited by blocking these receptors either by monoclonal antibodies or by antisense oligonucleotides. These data demonstrate that human osteogenic sarcoma cell lines are dependent on signaling through the IGF-I receptor for in vitro survival and proliferation. Furthermore, they suggest that modulation of the growth hormone/IGF-I axis may affect the growth of these tumors in vivo.

Original languageEnglish (US)
Pages (from-to)2803-2807
Number of pages5
JournalCancer Research
Volume54
Issue number10
StatePublished - May 15 1994
Externally publishedYes

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Osteosarcoma
Insulin-Like Growth Factor I
Cell Line
Growth
IGF Type 1 Receptor
Growth Hormone
Antisense Oligonucleotides
Bone Development
Neoplasms
Cell Survival
Monoclonal Antibodies
In Vitro Techniques
Bone and Bones
Survival

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Kappel, C. C., Velez-Yanguas, M. C., Hirschfeld, S., & Helman, L. J. (1994). Human osteosarcoma cell lines are dependent on insulin-like growth factor I for in vitro growth. Cancer Research, 54(10), 2803-2807.

Human osteosarcoma cell lines are dependent on insulin-like growth factor I for in vitro growth. / Kappel, Cynthia Chavez; Velez-Yanguas, Maria C.; Hirschfeld, Steven; Helman, Lee J.

In: Cancer Research, Vol. 54, No. 10, 15.05.1994, p. 2803-2807.

Research output: Contribution to journalArticle

Kappel, CC, Velez-Yanguas, MC, Hirschfeld, S & Helman, LJ 1994, 'Human osteosarcoma cell lines are dependent on insulin-like growth factor I for in vitro growth', Cancer Research, vol. 54, no. 10, pp. 2803-2807.
Kappel CC, Velez-Yanguas MC, Hirschfeld S, Helman LJ. Human osteosarcoma cell lines are dependent on insulin-like growth factor I for in vitro growth. Cancer Research. 1994 May 15;54(10):2803-2807.
Kappel, Cynthia Chavez ; Velez-Yanguas, Maria C. ; Hirschfeld, Steven ; Helman, Lee J. / Human osteosarcoma cell lines are dependent on insulin-like growth factor I for in vitro growth. In: Cancer Research. 1994 ; Vol. 54, No. 10. pp. 2803-2807.
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