Human osteoblast-like cells (MG63) proliferate on a bioactive glass surface

Neil Price, Stephen P. Bendall, Carmelita Frondoza, Riyaz H. Jinnah, David S. Hungerford

Research output: Contribution to journalArticle

Abstract

Bioglass®, a resorbable glass, previously has been evaluated as a bone graft substitute using cells of animal origin. Limited information is available on its effect on human cells. The objective of this study was to test the hypothesis that Bioglass® supports viability and proliferation of human bone cells. As a prototype of human bone cells, the osteoblast cell line MG63 was used and propagated on Bioglass® disks. MG63 cells also were seeded onto disks made of titanium (Ti-6Al-4V) and of cobalt chrome (Co-Cr- Mo) alloys. The number of viable cells recovered was similar for Bioglass®, titanium, and polystyrene control surfaces. Significantly fewer cells were recovered from CoCr (P < 0.05) compared to Bioglass®, Ti-6 Al-4v, and polystyrene surfaces. The proportion of cells undergoing DNA synthesis, estimated by thymidine uptake, was significantly greater on Bioglass® and titanium surfaces (P < 0.05) than on the CoCr surface. There were detectable differences in cell morphology on these biomaterials. Functional capacity was tested by assay of osteocalcin production and no differences were detectable among the different biomaterials. This study supports the hypothesis that 45S5 Bioglass® provides a favorable environment for human osteoblast proliferation and function. Bioglass® may have clinical potential as a bone graft substitute, a bioactive grout, or an implant coating for promoting bony ingrowth in uncemented prostheses.

Original languageEnglish (US)
Pages (from-to)394-400
Number of pages7
JournalJournal of Biomedical Materials Research
Volume37
Issue number3
DOIs
StatePublished - Dec 5 1997

Keywords

  • Biocompatibility
  • Bioglass®
  • Bone
  • Tissue culture

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

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