TY - JOUR
T1 - Human osteoblast-like cells (MG63) proliferate on a bioactive glass surface
AU - Price, Neil
AU - Bendall, Stephen P.
AU - Frondoza, Carmelita
AU - Jinnah, Riyaz H.
AU - Hungerford, David S.
PY - 1997/12/5
Y1 - 1997/12/5
N2 - Bioglass®, a resorbable glass, previously has been evaluated as a bone graft substitute using cells of animal origin. Limited information is available on its effect on human cells. The objective of this study was to test the hypothesis that Bioglass® supports viability and proliferation of human bone cells. As a prototype of human bone cells, the osteoblast cell line MG63 was used and propagated on Bioglass® disks. MG63 cells also were seeded onto disks made of titanium (Ti-6Al-4V) and of cobalt chrome (Co-Cr- Mo) alloys. The number of viable cells recovered was similar for Bioglass®, titanium, and polystyrene control surfaces. Significantly fewer cells were recovered from CoCr (P < 0.05) compared to Bioglass®, Ti-6 Al-4v, and polystyrene surfaces. The proportion of cells undergoing DNA synthesis, estimated by thymidine uptake, was significantly greater on Bioglass® and titanium surfaces (P < 0.05) than on the CoCr surface. There were detectable differences in cell morphology on these biomaterials. Functional capacity was tested by assay of osteocalcin production and no differences were detectable among the different biomaterials. This study supports the hypothesis that 45S5 Bioglass® provides a favorable environment for human osteoblast proliferation and function. Bioglass® may have clinical potential as a bone graft substitute, a bioactive grout, or an implant coating for promoting bony ingrowth in uncemented prostheses.
AB - Bioglass®, a resorbable glass, previously has been evaluated as a bone graft substitute using cells of animal origin. Limited information is available on its effect on human cells. The objective of this study was to test the hypothesis that Bioglass® supports viability and proliferation of human bone cells. As a prototype of human bone cells, the osteoblast cell line MG63 was used and propagated on Bioglass® disks. MG63 cells also were seeded onto disks made of titanium (Ti-6Al-4V) and of cobalt chrome (Co-Cr- Mo) alloys. The number of viable cells recovered was similar for Bioglass®, titanium, and polystyrene control surfaces. Significantly fewer cells were recovered from CoCr (P < 0.05) compared to Bioglass®, Ti-6 Al-4v, and polystyrene surfaces. The proportion of cells undergoing DNA synthesis, estimated by thymidine uptake, was significantly greater on Bioglass® and titanium surfaces (P < 0.05) than on the CoCr surface. There were detectable differences in cell morphology on these biomaterials. Functional capacity was tested by assay of osteocalcin production and no differences were detectable among the different biomaterials. This study supports the hypothesis that 45S5 Bioglass® provides a favorable environment for human osteoblast proliferation and function. Bioglass® may have clinical potential as a bone graft substitute, a bioactive grout, or an implant coating for promoting bony ingrowth in uncemented prostheses.
KW - Biocompatibility
KW - Bioglass®
KW - Bone
KW - Tissue culture
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U2 - 10.1002/(SICI)1097-4636(19971205)37:3<394::AID-JBM10>3.0.CO;2-C
DO - 10.1002/(SICI)1097-4636(19971205)37:3<394::AID-JBM10>3.0.CO;2-C
M3 - Article
C2 - 9368144
AN - SCOPUS:0031555503
VL - 37
SP - 394
EP - 400
JO - Journal of Biomedical Materials Research
JF - Journal of Biomedical Materials Research
SN - 1552-4973
IS - 3
ER -