Human oligodendrocytes and myelin in vitro to evaluate developmental neurotoxicity

Megan Chesnut, Thomas Hartung, Helena Hogberg, David Pamies

Research output: Contribution to journalReview articlepeer-review


Neurodevelopment is uniquely sensitive to toxic insults and there are concerns that environmental chemicals are contributing to widespread subclinical developmental neurotoxicity (DNT). Increased DNT evaluation is needed due to the lack of such information for most chemicals in common use, but in vivo studies recommended in regulatory guidelines are not practical for the large-scale screening of potential DNT chemicals. It is widely acknowledged that developmental neurotoxicity is a consequence of disruptions to basic processes in neurodevelopment and that testing strategies using human cell-based in vitro systems that mimic these processes could aid in prioritizing chemicals with DNT potential. Myelination is a fundamental process in neurodevelopment that should be included in a DNT testing strategy, but there are very few in vitro models of myelination. Thus, there is a need to establish an in vitro myelination assay for DNT. Here, we summarize the routes of myelin toxicity and the known models to study this particular endpoint.

Original languageEnglish (US)
Article number7929
JournalInternational journal of molecular sciences
Issue number15
StatePublished - Aug 1 2021


  • Developmental diseases
  • Developmental neurotoxicity
  • Myelin
  • Neurotoxicity
  • Oligodendrocytes
  • Organoid
  • Organotypic

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry


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