Human neutrophil activation and increased adhesion by various resuscitation fluids

Peter Rhee, Dennis Wang, Paul Ruff, Brenda Austin, Solenn DeBraux, Karen Wolcott, David Burris, Geoffrey Ling, Leon Sun

Research output: Contribution to journalArticle

Abstract

Objective: To determine whether activated neutrophils play a major role in secondary tissue injury after resuscitation in trauma. We hypothesized that human neutrophil activation and adhesion vary, depending on the type and amount of resuscitation fluid used. Setting: University-based research facility. Subjects: Ten healthy adult volunteers. Design: Whole blood from volunteers was serially diluted in polypropylene tubes with various resuscitation fluids. Fluids tested were phosphate-buffered saline, normal saline, lactated Ringer's solution, dextran, hespan, 5% human albumin, 25% human albumin, 3.5% hypertonic saline, and 7.5% hypertonic saline. Neutrophil activation (intracellular oxidative burst activity with dichlorofluorescin diacetate staining) and adhesion (integrin cell surface expression of CD18) were measured with flow cytometry (fluorescence-activated cell sorting). Blood was diluted with hypertonic saline by controlling for sodium content equal to normal saline. Measurements and Main Results: There was a significant dose-related increase in neutrophil oxidative burst activity as the result of dilution followed with crystalloid fluids and artificial colloids (dextran and hespan). The increase was 12-18 x baseline at the 75% dilution. The increase with 5% human albumin was only 2.2 x baseline, and 25% albumin did not demonstrate any increased intracellular activity. A similar significant increase in the neutrophil adhesion expression (CD18) occurred with artificial colloids (p < .05) and, to a lesser extent, with crystalloids, but not with albumin. Hypertonic saline caused a decrease in CD18 cell surface expression. Conclusions: This study suggests that the neutrophil activation and adhesion may vary, depending on the type of resuscitative fluid used. All artificial resuscitative fluids may not be similar or innocuous, as demonstrated by the dose-related increase in neutrophil activation and adhesion.

Original languageEnglish (US)
Pages (from-to)74-78
Number of pages5
JournalCritical Care Medicine
Volume28
Issue number1
DOIs
StatePublished - Jan 1 2000
Externally publishedYes

Fingerprint

Neutrophil Activation
Resuscitation
Albumins
Hydroxyethyl Starch Derivatives
Neutrophils
Respiratory Burst
Colloids
Dextrans
Flow Cytometry
Polypropylenes
Wounds and Injuries
Cell Adhesion
Integrins
Volunteers
Healthy Volunteers
Sodium
Phosphates
Staining and Labeling
Research

Keywords

  • Albumin
  • CD18
  • Dextran
  • Flow cytometry
  • Fluids
  • Hemodilution
  • Hespan
  • Hypertonic saline
  • Inflammation
  • Injury
  • Lactated Ringer's

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine

Cite this

Rhee, P., Wang, D., Ruff, P., Austin, B., DeBraux, S., Wolcott, K., ... Sun, L. (2000). Human neutrophil activation and increased adhesion by various resuscitation fluids. Critical Care Medicine, 28(1), 74-78. https://doi.org/10.1097/00003246-200001000-00012

Human neutrophil activation and increased adhesion by various resuscitation fluids. / Rhee, Peter; Wang, Dennis; Ruff, Paul; Austin, Brenda; DeBraux, Solenn; Wolcott, Karen; Burris, David; Ling, Geoffrey; Sun, Leon.

In: Critical Care Medicine, Vol. 28, No. 1, 01.01.2000, p. 74-78.

Research output: Contribution to journalArticle

Rhee, P, Wang, D, Ruff, P, Austin, B, DeBraux, S, Wolcott, K, Burris, D, Ling, G & Sun, L 2000, 'Human neutrophil activation and increased adhesion by various resuscitation fluids', Critical Care Medicine, vol. 28, no. 1, pp. 74-78. https://doi.org/10.1097/00003246-200001000-00012
Rhee, Peter ; Wang, Dennis ; Ruff, Paul ; Austin, Brenda ; DeBraux, Solenn ; Wolcott, Karen ; Burris, David ; Ling, Geoffrey ; Sun, Leon. / Human neutrophil activation and increased adhesion by various resuscitation fluids. In: Critical Care Medicine. 2000 ; Vol. 28, No. 1. pp. 74-78.
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AU - Wang, Dennis

AU - Ruff, Paul

AU - Austin, Brenda

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AU - Wolcott, Karen

AU - Burris, David

AU - Ling, Geoffrey

AU - Sun, Leon

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AB - Objective: To determine whether activated neutrophils play a major role in secondary tissue injury after resuscitation in trauma. We hypothesized that human neutrophil activation and adhesion vary, depending on the type and amount of resuscitation fluid used. Setting: University-based research facility. Subjects: Ten healthy adult volunteers. Design: Whole blood from volunteers was serially diluted in polypropylene tubes with various resuscitation fluids. Fluids tested were phosphate-buffered saline, normal saline, lactated Ringer's solution, dextran, hespan, 5% human albumin, 25% human albumin, 3.5% hypertonic saline, and 7.5% hypertonic saline. Neutrophil activation (intracellular oxidative burst activity with dichlorofluorescin diacetate staining) and adhesion (integrin cell surface expression of CD18) were measured with flow cytometry (fluorescence-activated cell sorting). Blood was diluted with hypertonic saline by controlling for sodium content equal to normal saline. Measurements and Main Results: There was a significant dose-related increase in neutrophil oxidative burst activity as the result of dilution followed with crystalloid fluids and artificial colloids (dextran and hespan). The increase was 12-18 x baseline at the 75% dilution. The increase with 5% human albumin was only 2.2 x baseline, and 25% albumin did not demonstrate any increased intracellular activity. A similar significant increase in the neutrophil adhesion expression (CD18) occurred with artificial colloids (p < .05) and, to a lesser extent, with crystalloids, but not with albumin. Hypertonic saline caused a decrease in CD18 cell surface expression. Conclusions: This study suggests that the neutrophil activation and adhesion may vary, depending on the type of resuscitative fluid used. All artificial resuscitative fluids may not be similar or innocuous, as demonstrated by the dose-related increase in neutrophil activation and adhesion.

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KW - Injury

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