Human neural stem cell transplantation reduces spontaneous recurrent seizures following pilocarpine-induced status epilepticus in adult rats

Kon Chu, Manho Kim, Keun Hwa Jung, Daejong Jeon, Soon Tae Lee, Juhyun Kim, Sang Wuk Jeong, Seung Up Kim, Sang Kun Lee, Hee Sup Shin, Jae Kyu Roh

Research output: Contribution to journalArticle

Abstract

Transplantation of neural stem cells (NSCs) can replace lost neurons and improve the functional deficits. Cell transplantation strategies have been tried in the epileptic disorder, but the effect of exogenous NSCs is unknown. In this study, we attempted to test the anti-epileptogenic effect of NSCs in adult rats with status epilepticus. Experimental status epilepticus was induced by lithium-pilocarpine injection, and β galactosidase-encoded human NSCs were transplanted intravenously on the next day of status epilepticus. Spontaneous recurrent seizures were monitored with Racine's seizure severity scale. Immunohistochemistry with anti-β gal, Tuj-1, NeuN, GFAP, CNPase, GluR2, parvalbumin, and GABA were performed and extracellular field excitatory postsynaptic potentials (fEPSP) were recorded. Human NSCs suppressed spontaneous recurrent seizure formation and transplanted NSCs were differentiated into GABA-immunoreactive interneurons in the damaged hippocampus. Amplitude of fEPSP in the hippocampal CA1 was reduced, which was reversed by picrotoxin. These findings suggest that NSCs could be differentiated into inhibitory interneurons and decrease neuronal excitability, which could prevent spontaneous recurrent seizure formation in adult rats with pilocarpine-induced status epilepticus.

Original languageEnglish (US)
Pages (from-to)213-221
Number of pages9
JournalBrain research
Volume1023
Issue number2
DOIs
StatePublished - Oct 15 2004
Externally publishedYes

Keywords

  • Epilepsy
  • Epileptogenesis
  • GABA
  • Inhibition
  • Interneuron
  • Neural stem cell

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Fingerprint Dive into the research topics of 'Human neural stem cell transplantation reduces spontaneous recurrent seizures following pilocarpine-induced status epilepticus in adult rats'. Together they form a unique fingerprint.

  • Cite this

    Chu, K., Kim, M., Jung, K. H., Jeon, D., Lee, S. T., Kim, J., Jeong, S. W., Kim, S. U., Lee, S. K., Shin, H. S., & Roh, J. K. (2004). Human neural stem cell transplantation reduces spontaneous recurrent seizures following pilocarpine-induced status epilepticus in adult rats. Brain research, 1023(2), 213-221. https://doi.org/10.1016/j.brainres.2004.07.045