TY - JOUR
T1 - Human-mouse somatic cell hybrids with single human chromosome (group E)
T2 - Link with thymidine kinase activity
AU - Migeon, Barbara Ruben
AU - Miller, Carol S.
PY - 1968
Y1 - 1968
N2 - Mouse somatic cells lacking thymidine kinase were mixed in culture with human diploid cells lacking hypoxanthine guanine phosphoribosyl transferase, and hybrid cells were isolated and maintained in a selective medium containing hypoxanthine, aminopterin, and thymidine. The hybrid cells at the time of isolation had karyotypes consisting predominantly of mouse chromosomes but with one human chromosome, a submetacentric member of group E, apparently giving thymidine kinase to the hybrid cell. However, after long-term propagation in the selective medium this chromosome has been lost, although cells continue to show thymidine kinase activity as demonstrated by the incorporation of 3H-thymidine into DNA in the hybrid cell. The hybrid cells have only mouse electrophoretic variants for glucose-6-phosphate dehydrogenase, lactate dehydrogenase, and malate dehydrogenase, suggesting that the human genetic loci for these enzymes are not represented in the hybrid genome and may be unlinked to that for thymidine kinase.
AB - Mouse somatic cells lacking thymidine kinase were mixed in culture with human diploid cells lacking hypoxanthine guanine phosphoribosyl transferase, and hybrid cells were isolated and maintained in a selective medium containing hypoxanthine, aminopterin, and thymidine. The hybrid cells at the time of isolation had karyotypes consisting predominantly of mouse chromosomes but with one human chromosome, a submetacentric member of group E, apparently giving thymidine kinase to the hybrid cell. However, after long-term propagation in the selective medium this chromosome has been lost, although cells continue to show thymidine kinase activity as demonstrated by the incorporation of 3H-thymidine into DNA in the hybrid cell. The hybrid cells have only mouse electrophoretic variants for glucose-6-phosphate dehydrogenase, lactate dehydrogenase, and malate dehydrogenase, suggesting that the human genetic loci for these enzymes are not represented in the hybrid genome and may be unlinked to that for thymidine kinase.
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U2 - 10.1126/science.162.3857.1005
DO - 10.1126/science.162.3857.1005
M3 - Article
C2 - 5698836
AN - SCOPUS:0014434397
VL - 162
SP - 1005
EP - 1006
JO - Science
JF - Science
SN - 0036-8075
IS - 3857
ER -