@article{9f70659f24bf4a999dfa7c3c3593cca9,
title = "Human monoclonal antibodies derived from memory B cells following live attenuated dengue virus vaccination or natural infection exhibit similar characteristics",
abstract = "The immunopathogenesis of severe dengue is poorly understood, but there is concern that induction of cross-reactive nonneutralizing antibodies by infection or vaccination may increase the likelihood of severe disease during a subsequent infection. We generated a total of 63 new human monoclonal antibodies to compare the B-cell response of subjects who received the National Institutes of Health live attenuated dengue vaccine rDEN1Δ30 to that of subjects following symptomatic primary infection with DENV1. Both infection and vaccination induced serum neutralizing antibodies and DENV1-reactive peripheral blood B cells, but the magnitude of induction was lower in vaccinated individuals. Serotype cross-reactive weakly neutralizing antibodies dominated the response in both vaccinated and naturally infected subjects. Antigen specificities were very similar, with a slightly greater percentage of antibodies targeting E protein domain I/II than domain III. These data shed light on the similarity of human B-cell response to live attenuated DENV vaccine or natural infection.",
keywords = "B cells, antibodies, antibody-dependent enhancement, dengue virus, human, hybridomas, neutralization",
author = "Smith, {Scott A.} and {De Alwis}, Ruklanthi and Nurgun Kose and Durbin, {Anna P.} and Whitehead, {Stephen S.} and {De Silva}, {Aravinda M.} and Crowe, {James E.}",
note = "Funding Information: 1Department of Medicine, 2Department of Pediatrics, 3Department of Pathology, Microbiology, and Immunology and 4The Vanderbilt Vaccine Center, Vanderbilt University Medical Center, Vanderbilt University, Nashville, Tennessee; 5Department of Microbiology and Immunology, University of North Carolina School of Medicine, Chapel Hill and 6Center for Immunization Research, Department of International Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, and 7Laboratory of Infectious Diseases, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland Funding Information: Financial Support. This work was supported by the NIH (grant U54 AI057157-the Southeastern Regional Center of Excellence for Emerging Infections and Biodefense; contract HHSN272200900042C to Alessandro Sette); grant K08 AI103038 to S. A. S.), and the Intramural Research Program of the National Institute of Allergy and Infectious Diseases, NIH. Potential conflicts of interest. All authors: No reported conflicts.",
year = "2013",
month = jun,
day = "15",
doi = "10.1093/infdis/jit119",
language = "English (US)",
volume = "207",
pages = "1898--1908",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "12",
}