Human milk glycosaminoglycans inhibit HIV glycoprotein gp120 binding to its host cell CD4 receptor

D. S. Newburg, R. J. Linhardt, S. A. Ampofo, Robert H Yolken

Research output: Contribution to journalArticle

Abstract

The binding of the HIV envelope glycoprotein, gp120, to its host cell receptor, CD4, is inhibited in a solid phase assay by a glycosaminoglycan of human milk; this binding is the essential first step in HIV infectivity. The human milk glycosaminoglycans were identified in this study. Pooled, fractionated human milk contained dermatan sulfate, heparin, heparan sulfate, and chondroitin sulfate. The ability of this glycosaminoglycan fraction to inhibit binding was unaffected by digestion with lytic enzymes specific for heparin, heparan sulfate and dermatan sulfate, but was lost when the milk fraction was treated with lytic enzymes specific for chondroitin sulfate. Furthermore, a purified milk fraction with high specific inhibitory activity contained chondroitin sulfate but not other glycosaminoglycans. This indicates that the ability of human milk to inhibit gp120 binding to CD4 may be attributed to chondroitin sulfate or to a chondroitin sulfate-like moiety rather than to other components of human milk. We speculate that this human milk glycosaminoglycan could limit the rate of postnatal vertical transmission of HIV in breast-fed infants of HIV-infected mothers.

Original languageEnglish (US)
Pages (from-to)419-424
Number of pages6
JournalJournal of Nutrition
Volume125
Issue number3
StatePublished - 1995
Externally publishedYes

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HIV Envelope Protein gp120
CD4 Antigens
glycosaminoglycans
Human Milk
chondroitin sulfate
breast milk
Glycosaminoglycans
Chondroitin Sulfates
glycoproteins
Glycoproteins
receptors
Dermatan Sulfate
Aptitude
Heparitin Sulfate
HIV
heparin
cells
Heparin
sulfates
Milk

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Human milk glycosaminoglycans inhibit HIV glycoprotein gp120 binding to its host cell CD4 receptor. / Newburg, D. S.; Linhardt, R. J.; Ampofo, S. A.; Yolken, Robert H.

In: Journal of Nutrition, Vol. 125, No. 3, 1995, p. 419-424.

Research output: Contribution to journalArticle

Newburg, D. S. ; Linhardt, R. J. ; Ampofo, S. A. ; Yolken, Robert H. / Human milk glycosaminoglycans inhibit HIV glycoprotein gp120 binding to its host cell CD4 receptor. In: Journal of Nutrition. 1995 ; Vol. 125, No. 3. pp. 419-424.
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