Human mesenchymal stem cells support megakaryocyte and pro-platelet formation from CD34+ hematopoietic progenitor cells

Linzhao Cheng, Pankaj Qasba, Padmavathy Vanguri, Mark A. Thiede

Research output: Contribution to journalArticle

Abstract

Megakaryocytopoiesis and thrombocytopoiesis result from the interactions between hematopoietic progenitor cells, humoral factors, and marrow stromal cells derived from mesenchymal stem cells (MSCs) or MSCs directly. MSCs are self-renewing marrow cells that provide progenitors for osteoblasts, adipocytes, chondrocytes, myocytes, and marrow stromal cells. MSCs are isolated from bone marrow aspirates and are expanded in adherent cell culture using an optimized media preparation. Culture-expanded human MSCs (hMSCs) express a variety of hematopoietic cytokines and growth factors and maintain long-term culture-initiating cells in long-term marrow culture with CD34+ hematopoietic progenitor cells. Two lines of evidence suggest that hMSCs function in megakaryocyte development. First, hMSCs express messenger RNA for thrombopoietin, a primary regulator for megakaryocytopoiesis and thrombocytopoiesis. Second, adherent hMSC colonies in primary culture are often associated with hematopoietic cell clusters containing CD41+ megakaryocytes. The physical association between hMSCs and megakaryocytes in marrow was confirmed by experiments in which hMSCs were copurified by immunoselection using an anti-CD41 antibody. To determine whether hMSCs can support megakaryocyte and platelet formation in vitro, we established a coculture system of hMSCs and CD34+ cells in serum-free media without exogenous cytokines. These cocultures produced clusters of hematopoietic cells atop adherent MSCs. After 7 days, CD41+ megakaryocyte clusters and pro-platelet networks were observed with pro-platelets increasing in the next 2 weeks. CD41+ platelets were found in culture medium and expressed CD62P after thrombin treatment. These results suggest that MSCs residing within the megakaryocytic microenvironment in bone marrow provide key signals to stimulate megakaryocyte and platelet production from CD34+ hematopoietic cells. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish (US)
Pages (from-to)58-69
Number of pages12
JournalJournal of Cellular Physiology
Volume184
Issue number1
DOIs
StatePublished - 2000

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Megakaryocytes
Hematopoietic Stem Cells
Platelets
Stem cells
Mesenchymal Stromal Cells
Blood Platelets
Thrombopoiesis
Bone Marrow
Cell culture
Stromal Cells
Coculture Techniques
Bone
Cells
Cytokines
Cell Culture Techniques
Thrombopoietin
Osteoblasts
Serum-Free Culture Media
Thrombin
Chondrocytes

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Human mesenchymal stem cells support megakaryocyte and pro-platelet formation from CD34+ hematopoietic progenitor cells. / Cheng, Linzhao; Qasba, Pankaj; Vanguri, Padmavathy; Thiede, Mark A.

In: Journal of Cellular Physiology, Vol. 184, No. 1, 2000, p. 58-69.

Research output: Contribution to journalArticle

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