Human marrow cd34+ and cd34+cd38- cells generate high levels of human hematofoiesis in lmmunodeficient mice

M. R. Miftm, N. Rottm, K. Gftta, G. Hofhn, I. Shnli, C. Civin

Research output: Contribution to journalArticlepeer-review

Abstract

Human bone marrow (BM) cells have been shown to engraft in immunodeficient mice. To determine whether engraftment derived from item cells and/or from more mature populations, we have transplanted adult human hematopoietic marrow populations enriched for stem cells. Sublethally irradiated NOD/LtSz seid/seid mice received human hematopoietic marrow cells intravenously, followed by intraperitoneal injections of recombinant human hematopoietic growth factors (rfaSCF, rhIL-3, rhGM-CSF and rhG-CSF) 3 times per week. Stem cell-enriched (purified CD34+ and CD34+CD38-) and total mononuclear cell populations were transplanted in separate experiments. One, 2 or 3 months after transplantation the mice were sacrificed, and cells were taken from BM, spleen and thymus. Row cytometric analysis IQQ-4 using murine monoclonal antibodies against g human leukocyte differentiation antigens was " n employed to quantify the frequencies of total human cells and the different hematopoietic lineages. PCR amplification of a specific human sequence and colony-forming assays confirmed hematopoietic cells were detected at all timepoints tested (figure). Human erythroid. myeloid and B-lymphoid cells were present. In addition, there was an expansion in pay 30 Day 60 DayJK) the number of CD34 cells. Thus, purified CD34+ CD34+CD38' provided long-term MONONUCLEAR CELLS muitilineage human engraftment, supporting the D34-t- CELLS hypothesis that adult human marrow stem cells CD34-.CD38-CELLS inthis system.

Original languageEnglish (US)
Pages (from-to)1047
Number of pages1
JournalExperimental Hematology
Volume24
Issue number9
StatePublished - 1996

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

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