TY - JOUR
T1 - Human liver debrisoquine 4-hydroxylase
T2 - test for specifity toward various monooxygenase substrates and model of the active site
AU - Wolff, Thomas
AU - Distlerath, Linda M.
AU - Worthington, Mark T.
AU - Guengerich, F. Peter
PY - 1987/5/1
Y1 - 1987/5/1
N2 - Polyclonal antibodies raised toward a debrisoquine 4-hydroxylating cytochrome P-450 species purified from rat liver (P-450UTA) were used to determine which monooxygenase reactions are linked to debrisoquine hydroxylation in human liver. Anti P-450UTA did not inhibit the oxidation of dimethylnitrosamine, morphine, diazepam, vinylidene chloride, trichloroethylene, benzo(a)pyrene and its 7.8-dihydrodiol, but was inhibitory for the hydroxylation of debrisoquine, (±)-bufuralol, lasiocarpine and monocrotaline. A model interpreting the substrate specificity of the human liver enzyme is presented.
AB - Polyclonal antibodies raised toward a debrisoquine 4-hydroxylating cytochrome P-450 species purified from rat liver (P-450UTA) were used to determine which monooxygenase reactions are linked to debrisoquine hydroxylation in human liver. Anti P-450UTA did not inhibit the oxidation of dimethylnitrosamine, morphine, diazepam, vinylidene chloride, trichloroethylene, benzo(a)pyrene and its 7.8-dihydrodiol, but was inhibitory for the hydroxylation of debrisoquine, (±)-bufuralol, lasiocarpine and monocrotaline. A model interpreting the substrate specificity of the human liver enzyme is presented.
KW - Cytochrome P-450
KW - Debrisoquine
KW - Human liver
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U2 - 10.1007/BF00296955
DO - 10.1007/BF00296955
M3 - Article
C2 - 3619650
AN - SCOPUS:0023219507
SN - 0340-5761
VL - 60
SP - 89
EP - 90
JO - Archives of Toxicology
JF - Archives of Toxicology
IS - 1-3
ER -