Human leukemia cells and mature lymphocytes induce platelet aggregation after removal of cell surface sialic acid.

Noriko Esumi, S. Todo, S. Imashuku

Research output: Contribution to journalArticle

Abstract

We examined platelet aggregating activity (PAA) of 5 human leukemia cell lines (HL-60, ML-1, HPB-ALL, RPMI-1788, K562), human mature lymphocytes and 2 human neuroblastoma lines (NCG, GOTO). Although intact cell suspensions of all leukemia cells and mature lymphocytes did not induce platelet aggregation, all cells exhibited PAA in both heparinized and citrated platelet rich plasma (PRP) following neuraminidase treatment (2 units/ml). In contrast, NCG and GOTO cells with PAA in intact cell suspensions were not affected by neuraminidase. PAA of HL-60 cells pre-cultured in the presence of tunicamycin (0.1-1.0 microgram/ml) to inhibit glycosylation decreased after neuraminidase treatment. Neuraminidase treatment had no effect on procoagulant activity of any of the cells examined. There was no difference in total sialic acid contents between human leukemia and neuroblastoma cells. These results suggest the cell surface glycoconjugates on hematopoietic cells play a role in PAA, and that sialic acid prevents their interaction with platelets.

Original languageEnglish (US)
Pages (from-to)767-773
Number of pages7
JournalActa Haematologica Japonica
Volume52
Issue number4
StatePublished - Jul 1989
Externally publishedYes

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N-Acetylneuraminic Acid
Platelet Aggregation
Leukemia
Lymphocytes
Blood Platelets
Neuraminidase
Neuroblastoma
Suspensions
Tunicamycin
Platelet-Rich Plasma
Glycoconjugates
HL-60 Cells
Glycosylation
Human Activities
Cell Line

ASJC Scopus subject areas

  • Hematology

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Human leukemia cells and mature lymphocytes induce platelet aggregation after removal of cell surface sialic acid. / Esumi, Noriko; Todo, S.; Imashuku, S.

In: Acta Haematologica Japonica, Vol. 52, No. 4, 07.1989, p. 767-773.

Research output: Contribution to journalArticle

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