Human immunodeficiency virus type 1 Vif is efficiently packaged into virions during productive but not chronic infection

Sandra Kao, Hirofumi Akari, Mohammad A. Khan, Markus Dettenhofer, Xiao Fang Yu, Klaus Strebel

Research output: Contribution to journalArticle

Abstract

Packaging of the human immunodeficiency virus type 1 Vif protein into virus particles is mediated through an interaction with viral genomic RNA and results in the association of Vif with the nucleoprotein complex. Despite the specificity of this process, calculations of the amount of Vif packaged have produced vastly different results. Here, we compared the efficiency of packaging of Vif into virions derived from acutely and chronically infected H9 cells. We found that Vif was efficiently packaged into virions from acutely infected cells (60 to 100 copies per virion), while packaging into virions from chronically infected H9 cells was near the limit of detection (four to six copies of Vif per virion). Superinfection by an exogenous Vif-defective virus did not rescue packaging of endogenous Vif expressed in the chronically infected culture. In contrast, exogenous Vif expressed by superinfection of wild-type virus was readily packaged (30 to 40 copies per virion). Biochemical analyses suggest that the differences in the relative packaging efficiencies were not due to gross differences in the steady-state distribution of Vif in chronically or acutely infected cells but are likely due to differences in the relative rates of de novo synthesis of Vif. Despite its low packaging efficiency, endogenously expressed Vif was sufficient to direct the production of viruses with almost wild-type infectivity. The results from our study provide novel insights into the biochemical properties of Vif and offer an explanation for the reported differences regarding Vif packaging.

Original languageEnglish (US)
Pages (from-to)1131-1140
Number of pages10
JournalJournal of Virology
Volume77
Issue number2
DOIs
StatePublished - Jan 2003

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Product Packaging
Human immunodeficiency virus 1
virion
Virion
packaging
HIV-1
Infection
infection
Superinfection
viruses
cells
nucleoproteins
Viruses
Defective Viruses
Nucleoproteins
Viral RNA
detection limit
pathogenicity
Limit of Detection
RNA

ASJC Scopus subject areas

  • Immunology

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Human immunodeficiency virus type 1 Vif is efficiently packaged into virions during productive but not chronic infection. / Kao, Sandra; Akari, Hirofumi; Khan, Mohammad A.; Dettenhofer, Markus; Yu, Xiao Fang; Strebel, Klaus.

In: Journal of Virology, Vol. 77, No. 2, 01.2003, p. 1131-1140.

Research output: Contribution to journalArticle

Kao, Sandra ; Akari, Hirofumi ; Khan, Mohammad A. ; Dettenhofer, Markus ; Yu, Xiao Fang ; Strebel, Klaus. / Human immunodeficiency virus type 1 Vif is efficiently packaged into virions during productive but not chronic infection. In: Journal of Virology. 2003 ; Vol. 77, No. 2. pp. 1131-1140.
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abstract = "Packaging of the human immunodeficiency virus type 1 Vif protein into virus particles is mediated through an interaction with viral genomic RNA and results in the association of Vif with the nucleoprotein complex. Despite the specificity of this process, calculations of the amount of Vif packaged have produced vastly different results. Here, we compared the efficiency of packaging of Vif into virions derived from acutely and chronically infected H9 cells. We found that Vif was efficiently packaged into virions from acutely infected cells (60 to 100 copies per virion), while packaging into virions from chronically infected H9 cells was near the limit of detection (four to six copies of Vif per virion). Superinfection by an exogenous Vif-defective virus did not rescue packaging of endogenous Vif expressed in the chronically infected culture. In contrast, exogenous Vif expressed by superinfection of wild-type virus was readily packaged (30 to 40 copies per virion). Biochemical analyses suggest that the differences in the relative packaging efficiencies were not due to gross differences in the steady-state distribution of Vif in chronically or acutely infected cells but are likely due to differences in the relative rates of de novo synthesis of Vif. Despite its low packaging efficiency, endogenously expressed Vif was sufficient to direct the production of viruses with almost wild-type infectivity. The results from our study provide novel insights into the biochemical properties of Vif and offer an explanation for the reported differences regarding Vif packaging.",
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