Human immunodeficiency virus type 1 strains of subtypes B and E replicate in cutaneous dendritic cell-T-cell mixtures without displaying subtype-specific tropism

Melissa Pope, Sarah S. Frankel, John R. Mascola, Alexandra Trkola, Frank Isdell, Deborah L. Birx, Donald S. Burke, D. H O David, John P. Moore

Research output: Contribution to journalArticle

Abstract

A report that genetic subtype E human immunodeficiency virus type 1 (HIV-1) strains display a preferential tropism for Langerhans cells (epidermal dendritic cells [DCs]) compared to genetic subtype B strains suggested a possible explanation for the rapid heterosexual spread of subtype E strains in Thailand (L. E. Soto-Ramirez et al., Science 271:1291-1293, 1996). In an independent system, we applied subtype E and B isolates to skin leukocytes, since skin is a relevant model for the histologically comparable surfaces of the vagina and ectocervix. Isolates of both HIV-1 subtypes infected DC-T-cell mixtures, and no subtype-specific pattern of infection was observed. Purified DCs did not support the replication of strains of either subtype B or E. Our findings do not support the conclusion that subtype E strains have a preferential tropism for DCs, suggesting that other explanations for the rapid heterosexual spread of subtype E strains in Asia should be considered.

Original languageEnglish (US)
Pages (from-to)8001-8007
Number of pages7
JournalJournal of Virology
Volume71
Issue number10
StatePublished - 1997
Externally publishedYes

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tropisms
Tropism
Langerhans Cells
dendritic cells
Human immunodeficiency virus 1
Dendritic Cells
HIV-1
T-lymphocytes
Heterosexuality
T-Lymphocytes
Skin
Vagina
Thailand
skin (animal)
Leukocytes
Langerhans cells
vagina
Infection
leukocytes
infection

ASJC Scopus subject areas

  • Immunology

Cite this

Pope, M., Frankel, S. S., Mascola, J. R., Trkola, A., Isdell, F., Birx, D. L., ... Moore, J. P. (1997). Human immunodeficiency virus type 1 strains of subtypes B and E replicate in cutaneous dendritic cell-T-cell mixtures without displaying subtype-specific tropism. Journal of Virology, 71(10), 8001-8007.

Human immunodeficiency virus type 1 strains of subtypes B and E replicate in cutaneous dendritic cell-T-cell mixtures without displaying subtype-specific tropism. / Pope, Melissa; Frankel, Sarah S.; Mascola, John R.; Trkola, Alexandra; Isdell, Frank; Birx, Deborah L.; Burke, Donald S.; David, D. H O; Moore, John P.

In: Journal of Virology, Vol. 71, No. 10, 1997, p. 8001-8007.

Research output: Contribution to journalArticle

Pope, M, Frankel, SS, Mascola, JR, Trkola, A, Isdell, F, Birx, DL, Burke, DS, David, DHO & Moore, JP 1997, 'Human immunodeficiency virus type 1 strains of subtypes B and E replicate in cutaneous dendritic cell-T-cell mixtures without displaying subtype-specific tropism', Journal of Virology, vol. 71, no. 10, pp. 8001-8007.
Pope, Melissa ; Frankel, Sarah S. ; Mascola, John R. ; Trkola, Alexandra ; Isdell, Frank ; Birx, Deborah L. ; Burke, Donald S. ; David, D. H O ; Moore, John P. / Human immunodeficiency virus type 1 strains of subtypes B and E replicate in cutaneous dendritic cell-T-cell mixtures without displaying subtype-specific tropism. In: Journal of Virology. 1997 ; Vol. 71, No. 10. pp. 8001-8007.
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