Human immunodeficiency virus type 1 neutralizing antibody serotyping using serum pools and an infectivity reduction assay

John R. Mascola, Mark K. Louder, Sonja R. Surman, Thomas C. Vancott, Xiao Fang Yu, James Bradac, Kevin R. Porter, Kenrad E. Nelson, Marc Girard, John G. McNeil, Francine E. McCutchan, Deborah L. Birx, Donald S. Burke

Research output: Contribution to journalArticle

Abstract

Classification of human immunodeficiency virus type 1 (HIV-1) by neutralization serotype may be important for the design of active and passive immunization strategies. Neutralizing antibody serotyping is hindered by the lack of standard reagents and assay format, and by the weak activity of many individual sera. To facilitate cross-clade neutralization analysis, we used an infectivity reduction assay (IRA) and selected clade-specific serum (or plasma) pools from subjects infected with clade B and E HIV-1, respectively. Several serum pools were utilized; some were selected for strong neutralizing activity against intraclade viruses and others were derived from conveniently available samples. Against a panel of 51 clade B and E viruses, serum pools displayed strong neutralization of most intraclade viruses and significantly diminished cross-clade neutralization. Results were confirmed against a blinded panel of 20 viruses. The data indicate that the phylogenetic classification of virus subtypes B and E corresponds to two distinct neutralization serotypes. This approach to neutralizing antibody serotyping may be useful in defining the antigenic relationship among viruses from other clades.

Original languageEnglish (US)
Pages (from-to)1319-1328
Number of pages10
JournalAIDS research and human retroviruses
Volume12
Issue number14
DOIs
StatePublished - Sep 20 1996

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ASJC Scopus subject areas

  • Immunology
  • Virology
  • Infectious Diseases

Cite this

Mascola, J. R., Louder, M. K., Surman, S. R., Vancott, T. C., Yu, X. F., Bradac, J., Porter, K. R., Nelson, K. E., Girard, M., McNeil, J. G., McCutchan, F. E., Birx, D. L., & Burke, D. S. (1996). Human immunodeficiency virus type 1 neutralizing antibody serotyping using serum pools and an infectivity reduction assay. AIDS research and human retroviruses, 12(14), 1319-1328. https://doi.org/10.1089/aid.1996.12.1319