Human immune response directed against Plasmodium falciparum heat shock-related proteins

N. Kumar, Y. Zhao, P. Graves, J. P. Folgar, L. Maloy, H. Zheng

Research output: Contribution to journalArticle

Abstract

Heat shock-related stress proteins present in all eucaryotes and procaryotes have been shown to be immune targets in a broad range of infections. We have analyzed sera from people exposed primarily to Plasmodium falciparum for specific antibodies against two heat shock-related proteins (proteins similar to the heat shock protein with a molecular weight of 75,000 [Pfhsp] and a glucose-regulated protein with a molecular weight of 72,000 [Pfgrp]). In an immunoprecipitation analysis with metabolically labeled parasites and synthetic peptides in an enzyme-linked immunosorbent assay, specific antibodies against Pfhsp and Pfgrp were detected in the sera of these individuals. Sera from people exposed to a different human malarial parasite, Plasmodium vivax, did not react with the peptides in an enzyme-linked immunosorbent assay. Southern blot analysis with DNA isolated from P. falciparum from different geographical locations showed a conservation of genes for these stress proteins; thus, they are likely to be immune targets in various endemic areas. Lymphocytes from two tested immune donors responded in proliferation assays to purified Pfhsp and Pfgrp and purified recombinant proteins. However, a similar response was also seen in lymphocytes from nonimmune individuals and has raised questions pertaining to a generalized responsiveness of lymphocytes to some common determinants present in heat shock-related proteins in various pathogens.

Original languageEnglish (US)
Pages (from-to)1408-1414
Number of pages7
JournalInfection and immunity
Volume58
Issue number5
StatePublished - Jan 1 1990

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Human immune response directed against Plasmodium falciparum heat shock-related proteins'. Together they form a unique fingerprint.

  • Cite this

    Kumar, N., Zhao, Y., Graves, P., Folgar, J. P., Maloy, L., & Zheng, H. (1990). Human immune response directed against Plasmodium falciparum heat shock-related proteins. Infection and immunity, 58(5), 1408-1414.