Human IgM monoclonal antibody 16.88: Pharmacokinetics and immunogenicity in colorectal cancer patients

Hidde J. Haisma, Herbert M. Pinedo, Mieke A P Kessel, Monique Van Muijen, Jan C. Roos, Marian A B D Plaizier, Harrie J M Martens, Robert DeJager, Epie Boven

Research output: Contribution to journalArticle

Abstract

Twenty colorectal cancer patients were given an intravenous injection of human IgM monoclonal antibody (MAb) 16.88 (8 mg) conjugated to 131I for tumor localization. After a 2-week interval, a second injection with 200, 500, or 1000 mg of unlabeled antibody added was given to groups of five patients each. At the end of the 2-hour infusion, 66% of the radioactivity remained in the circulation. Blood clearance of the 131I-labeled MAb 16.88 was biphasic with a mean half-life (T1/2α) of 12 hours and T1/2β of 45 hours. Clearance rate was 0.09 L/hour. More than 90% of the 131I in serum was protein bound, with an immunoreactive fraction of 80% in the first 48 hours. Size exclusion chromatography indicated no degradation products other than 131I in serum and urine. The urinary excretion rate of 131I increased to 1.5% of the dose per hour at 24 hours, with 50% of the dose excreted in 34 hours. The pharmacokinetic profile of 131I-labeled MAb 16.88 was neither influenced by the total protein dose of antibody administered nor affected by specific uptake in tumor tissue in individual patients, as determined on early immunoscintigrams. The larger antibody doses showed a slightly slower excretion of 131I. The assays applied to determine immunogenicity were enzyme-linked immunosorbent assay, radioimmunoassay, and the dot-blot assay. They had sensitivities ranging from 5 ng/mL to 0.5 μg/mL for goat or rabbit antihuman IgM. The assays did not reveal antihuman antibody responses.

Original languageEnglish (US)
Pages (from-to)1813-1819
Number of pages7
JournalJournal of the National Cancer Institute
Volume83
Issue number24
StatePublished - Dec 18 1991
Externally publishedYes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging

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    Haisma, H. J., Pinedo, H. M., Kessel, M. A. P., Van Muijen, M., Roos, J. C., Plaizier, M. A. B. D., Martens, H. J. M., DeJager, R., & Boven, E. (1991). Human IgM monoclonal antibody 16.88: Pharmacokinetics and immunogenicity in colorectal cancer patients. Journal of the National Cancer Institute, 83(24), 1813-1819.