Human HPK1, a novel human hematopoietic progenitor kinase that activates the JNK/SAPK kinase cascade

Mickey C.T. Hu, Wan R. Qiu, Xiaoping Wang, Christian F. Meyer, Tse Hua Tan

Research output: Contribution to journalArticlepeer-review

Abstract

The c-Jun amino-terminal kinases (JNKs)/stress-activated protein kinases (SAPKs) play a crucial role in stress responses in mammalian cells. The mechanism underlying this pathway in the hematopoietic system is unclear, but it is a key in understanding the molecular basis of blood cell differentiation. We have cloned a novel protein kinase, termed hematopoietic progenitor kinase 1 (HPK1), that is expressed predominantly in hematopoietic cells, including early progenitor cells. HPK1 is related distantly to the p21(Cdc42/Rac1)-activated kinase (PAK) and yeast STE20 implicated in the mitogen-activated protein kinase (MAPK) cascade. Expression of HPK1 activates JNK1 specifically, and it elevates strongly AP-1-mediated transcriptional activity in vivo. HPK1 binds and phosphorylates MEKK1 directly, whereas JNK1 activation by HPK1 is inhibited by a dominant-negative MEKK1 or MKK4/SEK mutant. Interestingly, unlike PAK65, HPK1 does not contain the small GTPase Rac1/Cdc42-binding domain and does not bind to either Rac1 or Cdc42, suggesting that HPK1 activation is Rac1/Cdc42-independent. These results indicate that HPK1 is a novel functional activator of the JNK/SAPK signaling pathway.

Original languageEnglish (US)
Pages (from-to)2251-2264
Number of pages14
JournalGenes and Development
Volume10
Issue number18
DOIs
StatePublished - 1996
Externally publishedYes

Keywords

  • JNK/SAPK
  • MAP kinase
  • hematopoietic cells
  • protein kinase
  • signal transduction

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Fingerprint

Dive into the research topics of 'Human HPK1, a novel human hematopoietic progenitor kinase that activates the JNK/SAPK kinase cascade'. Together they form a unique fingerprint.

Cite this